Placental growth hormone

Reagents were obtained from Life Technologies Corporation (Carlsbad, CA, United States) unless otherwise indicated. Estrone (E1), estradiol (E2), estriol (E2), P4, CRT, rifampicin (RIF), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-(3,4-dichlorobenzyl)oxime (CITCO), chenodeoxycholic acid (CDCA), piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), phenylmethylsulfonyl fluoride (PMSF), sucrose, and dimethyl sulfoxide (DMSO) were obtained from Sigma Aldrich (St. Louis, MO, United States). Placental growth hormone (pGH) was purchased from R&D Systems (Minneapolis, MN, United States). QualGro^™ Seeding, QualGro^™ Culture, and QualGro^™ Induction media were obtained from BioIVT (Durham, NC); Matrigel Matrix and Corning Biocoat^™ collagen I coated plates were purchased from Corning (Corning, NY, United States). Enzyme-linked immunosorbent assay (ELISA) kits for E2, E3, P4, and CRT were purchased from Cayman Chemical (Ann Arbor, MI, United States). Ethylenediaminetetraacetic acid (EDTA) and digitonin were obtained from EMD Millipore (Burlington, MA, United States).

Reagents were obtained from Life Technologies Corporation (Carlsbad, CA) unless otherwise indicated. Dimethyl sulfoxide (DMSO), rifampin (RIF), MDZ, 1-hydroxy-midazolam (1-OH-MDZ), α-Hydroxymidazolam-d4, NIF, dehydro-NIF, BUP, nor-BUP, norbuprenorphine-d3, estrone (E1), E2, estriol (E3), P4, and CRT were purchased from Sigma-Aldrich (St. Louis, MO). E1, E2, E3, P4, CRT, and RIF stock solutions were prepared in DMSO. Placental growth hormone (pGH) was obtained from R&D Systems (Minneapolis, MN), and stock solution was prepared in pure water. Corning Biocoat™ collagen I coated plates and Matrigel^® Matrix were purchased from Corning (Corning, NY). Dehydronifedipine-d6 was obtained from Toronto Research Chemicals (Toronto, ON, Canada). QualGro™ Seeding, QualGro™ Culture, and QualGro™ Induction media were purchased from BioIVT (Durham, NC).