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Anti cd1d 1b1

Manufactured by BD

Anti-CD1d (1B1) is a laboratory reagent used for the identification and characterization of CD1d-expressing cells in flow cytometry applications. CD1d is a non-classical major histocompatibility complex (MHC) class I-like molecule that presents lipid and glycolipid antigens to natural killer T (NKT) cells.

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2 protocols using anti cd1d 1b1

1

Multiparameter Flow Cytometry Analysis

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For flow cytometry, cells were stained with following antibodies: anti-CD3ε (145-2C11), anti-CD8 (53-6.7), anti-CD4 (GK1.5), anti-CD24 (M1/69), anti-CD45.1 (A20), anti-CD45.2 (104), anti-B220 (RA3-6B2), anti-NKG2D (CX5), anti-IL-2Rβ (TM-β1), anti-CD94 (18d3), anti-IFN-γ (XMG1.2), anti-IL-4 (11B11), and anti-T-bet (4B10) were purchased from BioLegend. Anti-NK1.1 (PK136), anti-TCRβ (H57-597), anti-CD44 (IM7), anti-IgG1 (A85-1), anti-CD1d (1B1), and anti-CD90.2 (53-2.1) were obtained from BD Biosciences. Anti-IL-17 (ebio17B7) and anti-Rorγt (AFKJS-9) were purchased from eBioscience. Anti-PLZF (D-9) was obtained from Santa Cruz Biotechnology. Anti-CXCR3 (220803) was purchased from R&D Systems. Anti-S100a6 (EPNCIR121) was obtained from Abcam. Anti-CD16/CD32 (2.4G2) was acquired from Bio-Xcell (Malaysia).
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry, cells were stained with following antibodies: anti-CD3ε (145-2C11), anti-CD8 (53-6.7), anti-CD4 (GK1.5), anti-CD24 (M1/69), anti-CD45.1 (A20), anti-CD45.2 (104), anti-B220 (RA3-6B2), anti-NKG2D (CX5), anti-IL-2Rβ (TM-β1), anti-CD94 (18d3), anti-IFN-γ (XMG1.2), anti-IL-4 (11B11), and anti-T-bet (4B10) were purchased from BioLegend. Anti-NK1.1 (PK136), anti-TCRβ (H57-597), anti-CD44 (IM7), anti-IgG1 (A85-1), anti-CD1d (1B1), and anti-CD90.2 (53-2.1) were obtained from BD Biosciences. Anti-IL-17 (ebio17B7) and anti-Rorγt (AFKJS-9) were purchased from eBioscience. Anti-PLZF (D-9) was obtained from Santa Cruz Biotechnology. Anti-CXCR3 (220803) was purchased from R&D Systems. Anti-S100a6 (EPNCIR121) was obtained from Abcam. Anti-CD16/CD32 (2.4G2) was acquired from Bio-Xcell (Malaysia).
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