Myoblast differentiation was examined according to previously published protocols [18 (link)], and samples were imaged on INCell Analyzer 6000 (GE Healthcare), equipped with 10×/0.45 NA Plan Apo objective and 2048 × 2048 sCMOS camera. Twenty-five fields with five z-planes were acquired. Individual focal plane image for each z-series was automatically selected based on sharpness. Image analysis algorithm and PCR is described in Supplementary Methods .
Plan apo
Manufactured by GE Healthcare
The Plan Apo is a high-performance optical lens designed for use in laboratory equipment. It offers superior image quality and clarity, with a flat field of view and reduced distortion. The lens is suitable for a range of applications that require precise, high-resolution imaging.
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Lab products found in correlation
2 protocols using plan apo
1
Myoblast Differentiation Imaging Protocol
2
Automated Imaging of Tau Aggregates in HEK293T Cells
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HEK293T cells treated with virus expressing either tau RD S320F, S320F_I328S, or P301S were plated at 10,000 cells per well in media (10% FBS, 1% Pen/Strep, 1% GlutaMax in Dulbecco’s modified Eagle’s medium) in a 96-well clear bottom plate (Corning, Product # 3603). After 24 h, cells were stained with Hoechst33342 and Wheat Germ Agglutinin at a final concentration of 5 μg/mL in media for 10 min at 37 °C, protected from light. The staining solution was removed and substituted with 1x PBS afterward. The plate was placed on an IN Cell 6000 Analyzer (GE Healthcare) with a heated stage, and 50 fields of view were imaged under 4′,6-diamidino-2-phenylindole (DAPI), and FITC channels at ×60 magnification (Nikon ×60/0.95, Plan Apo, Corr Collar 0.11–0.23, CFI/60 lambda). Images were exported as TIFF files for downstream analysis.
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