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Rea747

Manufactured by Miltenyi Biotec
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Sourced in Germany

The REA747 is a laboratory equipment product from Miltenyi Biotec. It is designed for the isolation and separation of cells and biological samples. The core function of the REA747 is to enable efficient and reliable cell separation and purification.

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Lab products found in correlation

Rea804, by Miltenyi Biotec (2 mentions) Rea794, by Miltenyi Biotec (2 mentions) Flowlogic, by Miltenyi Biotec (2 mentions) Rea897, by Miltenyi Biotec (2 mentions) Rea293, by Miltenyi Biotec (2 mentions) Macsquant 10, by Miltenyi Biotec (2 mentions)

2 protocols using rea747

1

Immunophenotyping of Adipose-Derived Stem Cells

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Surface marker expression was analyzed by flow cytometry. ASCs were used from 4 donors. Cells were incubated at for 4 °C for 15 min in the dark with the following antibodies: CD45 (1:50, REA747), CD73 (1:50, REA804), CD90 (1:50, REA897), CD105 (1:50, REA794) as well as the isotype control (1:50, REA293) (all from Miltenyi Biotec, Germany). Flow cytometry measurement was done on a MACSQuant10 (Miltenyi Biotech) and data was analyzed using FlowLogic (Miltenyi Biotech). A minimum of 10,000 events was analyzed for each sample.
Kaniowska D., Wenk K., Rademacher P., Weiss R., Fabian C., Schulz I., Guthardt M., Lange F., Greiser S., Schmidt M., Braumann U.D., Emmrich F., Koehl U, & Jaimes Y. (2022). Extracellular Vesicles of Mesenchymal Stromal Cells Can be Taken Up by Microglial Cells and Partially Prevent the Stimulation Induced by β-amyloid. Stem Cell Reviews and Reports, 18(3), 1113-1126.
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2

Flow Cytometric Characterization of ASCs

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Surface marker expression was analyzed by ow cytometry. ASCs were used from 4 donors. Cells were incubated at for 4°C for 15 minutes in the dark with the following antibodies: CD45 (1:50, REA747), CD73 (1:50, REA804), CD90 (1:50, REA897), CD105 (1:50, REA794) as well as the isotype control (1:50, REA293) (all from Miltenyi Biotec, Germany). Flow cytometry measurement was done on a MACSQuant10 (Miltenyi Biotech) and data was analyzed using FlowLogic (Miltenyi Biotech). A minimum of 10,000 events was analyzed for each sample.
Kaniowska D., Wenk K., Rademacher P., Weiß R., Fabian C., Schulz I., Guthardt M., Lange F., Greiser S., Schmidt M., Braumann U., Emmrich F., Koehl U., & Jaimes Y. (2021). Extracellular Vesicles of Mesenchymal Stromal Cells Can be Taken up by Microglial Cells and Partially Prevent The Stimulation Induced by β-Amyloid.
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