Human peripheral blood mononuclear cells (PBMCs) were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer's protocol. Briefly, cells were incubated with NK Cell Biotin-Antibody Cocktail and NK Cell MicroBead Cocktail to label non-NK cells, followed by loading onto a LS Separation column (Miltenyi Biotec). The cell suspension was then placed in the magnetic field of a MACS Separator to separate labeled from non-labeled cells. The isolated human NK cells were subsequently cultured in Roswell Park Memorial Institute (RPMI) 1640 media supplemented with 10% fetal bovine serum, 1x penicillin/streptomycin and 100 U/mL IL-2 at 37°C in 5% CO 2 . The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
Nk cell biotin antibody cocktail
Manufactured by Miltenyi Biotec
The NK Cell Biotin-Antibody Cocktail is a lab equipment product designed for the isolation of natural killer (NK) cells from human peripheral blood mononuclear cells (PBMCs). The cocktail contains a mixture of biotin-conjugated monoclonal antibodies that specifically bind to surface antigens expressed on NK cells, enabling their separation from other cell types using magnetic separation techniques.
Automatically generated - may contain errors
Lab products found in correlation
Pe vio 770 anti human cd56, by Miltenyi Biotec (2 mentions)
Rea196 clone, by Miltenyi Biotec (2 mentions)
Fitc anti human cd3, by Miltenyi Biotec (2 mentions)
Rea613 clone, by Miltenyi Biotec (2 mentions)
Nk cell microbead cocktail, by Miltenyi Biotec (2 mentions)
Ls separation column, by Miltenyi Biotec (2 mentions)
Ficoll paque plus, by GE Healthcare (2 mentions)
Nk cell isolation kit, by Miltenyi Biotec (2 mentions)
2 protocols using nk cell biotin antibody cocktail
1
Isolation and Culture of Human NK Cells
2
Isolation and Culture of Primary Human NK Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human peripheral blood mononuclear cells (PBMCs) were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. Briefly, cells were incubated with NK Cell Biotin-Antibody Cocktail and NK Cell MicroBead Cocktail to label non-NK cells, followed by loading onto a LS Separation column (Miltenyi Biotec). The cell suspension was then placed in the magnetic field of a MACS Separator to separate labeled from non-labeled cells. The isolated human NK cells were subsequently cultured in Roswell Park Memorial Institute (RPMI) 1640 media supplemented with 10% fetal bovine serum, 1x penicillin/streptomycin and 100 U/mL IL-2 at 37°C in 5% CO2. The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!