Human adipogenesis rt2 profiler pcr array

To investigate a wide spectrum of genes involved in lipid metabolism the Human Adipogenesis RT² Profiler PCR Array (Qiagen) was used. 84 “metabolic” genes were analyzed in addition to positive control genes (beta actin and Ribosomal protein). Real-time PCR was performed with 7500 Fast Real-Time (Applied Biosystems) using Sybr green detection. Results were analyzed by RT² Profiler PCR Array Data Analysis. Transcriptional levels of genes were shown as fold change vs Healthy Donors, values >1.50 or <0.5 were considered significantly modified. All dataset was submitted in Geo Repository http://www.ncbi.nlm.nih.gov/geo. Accession number: GSE54432

The expression of 84 metabolic genes involved lipid metabolism was analyzed in HepG2 cells treated with MOES 2.5 mg/ml, and mouse liver tissues exposed to mol-miR pool for 5 weeks. Analysis was performed on the cDNA obtained from total RNA using Human Adipogenesis RT² Profiler PCR Array (PAHS-049Z; Qiagen) and Mouse Adipogenesis RT² Profiler PCR Array (PAMM-049Z; Qiagen), respectively. A 7500 Fast Real-Time kit (Applied Biosystems) with SYBR Green detection (Hydra SYBR qPCR Master Mix, BIOLAB) was used according to the manufacturer’s instructions. Data elaboration was performed using the RT² Profiler PCR Array Data Analysis software. Transcriptional levels were shown as fold change (HFD vs. ND; HFD + mol-miR pool vs. ND; HFD group vs. HFD + mol-miR pool). Values > 1.50 units or <0.5 units were considered significant. All dataset was submitted to Geo Repository http://www.ncbi.nlm.nih.gov/geo. Accession number: GSE125344 (human), GSE125346 (mouse).