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Chromasolv grade

Manufactured by Merck Group
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Sourced in Italy, Germany, United States

Chromasolv grade is a line of high-purity solvents and reagents designed for use in analytical and chromatographic applications. These products are manufactured to stringent quality standards to ensure consistent performance and reliability.

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Lab products found in correlation

Acetic acid, by Merck Group (2 mentions) Isobutyric acid, by Merck Group (1 mentions) Valeric acid, by Merck Group (1 mentions) 5971 single quadrupole mass spectrometer, by Agilent Technologies (1 mentions) Isovaleric acid, by Merck Group (1 mentions) Propionic acid, by Merck Group (1 mentions) Butyric acid, by Merck Group (1 mentions) 2h3 acetic, by Merck Group (1 mentions) 2h3 propionic, by Merck Group (1 mentions) 2h7 iso butyric, by Merck Group (1 mentions) 2h9 iso valeric, by Merck Group (1 mentions) Milliq water 18 mω, by Merck Group (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Gc ms system, by Agilent Technologies (1 mentions) Acquity uplc beh c18 column, by Waters Corporation (1 mentions) Formic acid, by Merck Group (1 mentions) Acetonitrile, by AccuStandard (1 mentions) Brodifacoum, by AccuStandard (1 mentions) Diphacinone, by AccuStandard (1 mentions)

Close spelling variants of this product

Chromasolv-grade methanol and water Chromasolv® grade ethyl acetate LC–MS Chromasolv grade CHROMASOLV® gradient grade Chromasolv-grade methanol Chromasolv-grade acetonitrile Chromasolv HPLC grade Chromasolv

4 protocols using chromasolv grade

1

GC-MS Analysis of Short-Chain Fatty Acids

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The SCFAs’ analysis was performed by Agilent GC-MS system composed with 5971 single quadrupole mass spectrometer, 5890 gas-chromatograph and 7673 autosampler. Methanol and tert-Butyl methyl ether (Chromasolv grade), Sodium bicarbonate and Hydrochloric acid (Reagent grade), [2H3]Acetic, [2H3]Propionic, [2H7]iso-Butyric and [2H9]iso-Valeric, used as internal standards (ISTDs), acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid (analytical standards grade) were purchased by Sigma-Aldrich (Milan, Italy). MilliQ water 18 MΩ was obtained from Millipore's Simplicity system (Milan, Italy).
Niccolai E., Baldi S., Ricci F., Russo E., Nannini G., Menicatti M., Poli G., Taddei A., Bartolucci G., Calabrò A.S., Stingo F.C, & Amedei A. (2019). Evaluation and comparison of short chain fatty acids composition in gut diseases. World Journal of Gastroenterology, 25(36), 5543-5558.
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2

Specialized Metabolite Extraction and Analysis

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Specialized metabolites were extracted as described in Sipari et al. [13 (link)] and analyzed with the same instrumentation as the lipophilic metabolites. Samples were analyzed both in positive (ESI +) and negative (ESI −) ionization mode. The mass range (m/z) was set to 100–1500 in positive and 100–1000 in negative mode. The compounds were separated on an Acquity UPLC BEH C18 column (1.7 µm, 50 × 2.1 mm, Waters, Ireland). The injection volume was 2 µL, oven temperature was 40 °C, and tray temperature was 10 °C. The mobile phases consisted of (A) H2O and (B) acetonitrile (Chromasolv grade, Sigma-Aldrich, Steinheim, Germany) both containing 0.1% formic acid (Sigma-Aldrich, Steinheim, Germany). A linear gradient started from 95% A and proceeded to 10% in 5 min. The eluent composition was changed back to 95% at 5.1 min and left to equilibrate for 0.9 min, with a total analysis time of 6 min. Data processing with MassLynx and the identification of metabolites were performed as described for lipophilic metabolites. The relative levels of the specialized metabolites were calculated by normalizing the analyte peak area by the peak area of the internal standard, 4-methyl-umbelliferone, and the fresh weight of the sample.
Sipari N., Lihavainen J, & Keinänen M. (2022). Metabolite Profiling of Paraquat Tolerant Arabidopsis thaliana Radical-induced Cell Death1 (rcd1)—A Mediator of Antioxidant Defence Mechanisms. Antioxidants, 11(10), 2034.
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3

Competitive Ionization of Rodenticides and Drugs

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A total of six rodenticides were examined in this work, brodifacoum, bromadiolone, chlorophacinone, difenacoum, diphacinone, and pindone, and were purchased as 0.1 mg/mL solutions in methanol or acetonitrile from AccuStandard (New Haven, CT, USA). Samples were further diluted, as required, using methanol (Chromasolv Grade, Sigma-Aldrich, St. Louis, MO, USA). For the competitive ionization studies, 1 mg/mL solutions of ABFUBINACA, cocaine, heroin, and methamphetamine were used either neat or diluted in methanol. AB-FUBINACA was acquired from Cayman Chemical (Ann Arbor, MI, USA) while the remaining drug standards were acquired from Cerilliant (Round Rock, TX, USA). All samples were pipetted (1–5 μL) onto the center of the desorbed area of the PTFE-coated fiberglass wipes (DSA Detection, North Andover, MA, USA) for analysis. McCormick gourmet organic Oregano was purchased from a local grocery store. Seized plant material was provided by Maryland State Police Forensic Sciences Division and was known to contain 4-fluoro ADB, MMB-FUBINACA, and ADB-FUBINACA.
Robinson E.L, & Sisco E. (2018). Detection of Brodifacoum and other Rodenticides in Drug Mixtures using Thermal Desorption Direct Analysis in Real Time Mass Spectrometry (TD-DART-MS). Journal of forensic sciences, 64(4), 1026-1033.
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4

Photochemical Damage Analysis Reagents

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Methanol (MeOH) >99.9 % purity (CHROMASOLV grade) was purchased from Sigma-Aldrich and LC-MS grade acetonitrile (ACN) from Carlo Erba. Triethylammonium acetate (TEAA, 1 M in water), tris(hydroxymethyl)aminomethane (Tris), ethylene diamine tetraacetic acid (EDTA), sucrose, Triton-X100, sodium dodecyl sulfate (SDS), chloroacetaldehyde and 1,N 6 -etheno-2'-deoxyadenosine (dAdo) were from Sigma (Saint-Quentin-Fallavier, France). Reagents and solvents for synthesis
(5'-dimethoxytrityl-3'-phosphoramidite-thymidine, dimethoxytrityl chloride, acetic anhydride, acetic acid, pyridine, ethyl-S-triazol, iodine, ammonium hydroxide) were of the highest purity available and purchased form Aldrich (Saint-Quentin-Fallavier, France). ( 13 C10, 98% 15 N2, 96-98%)-thymidine was from Cambridge Isotope Laboratories. [ 15 N5]-dAdo was synthetized as previously reported (35) . Thymidylyl-(3'-5')-thymidine (TpT) was prepared by phosphodiester synthesis and all photoproducts of dinucleoside monophosphates were obtained as previously reported (23) .
Reynaud N., Belz L., Béal D., Bacqueville D., Duplan H., Géniès C., Questel E., Josse G, & Douki T. (2022). DNA photoproducts released by repair in biological fluids as biomarkers of the genotoxicity of UV radiation. Analytical and bioanalytical chemistry, 414(26).
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