All solvents used were of reagent grade. Tryptone and soy peptone were purchased from Biolife, Milano, Italy. Agar was purchased from Sigma-Aldrich St. Louis, MO, USA product of Spain. Sodium cloride, D(+)–Glucose, and di potassium hydrogen phosphate trihydrate were purchased from Merck, Darmstadt, Germany. DMSO was purchased from Riedel–de Haen (Seelze, Germany). Melting points were measured in open tubes with a Stuart Scientific apparatus and are uncorrected. A UV–1600 PC series spectrophotometer of VWR international GmbH, Darmstadt, Germany was used to obtain electronic absorption spectra. ATR-FTIR spectra in the region of 4000–370 cm–1 were obtained with a Cary 670 FTIR spectrometer, Agilent Technologies Agilent Technologies. XRF measurement was carried out using an Am–241 radio isotopic source (exciting radiation 59.5 keV).
Tryptone
Manufactured by Biolife
Sourced in Italy
Tryptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of casein. It is commonly used as a nutrient source in microbiology and cell culture applications, providing essential growth factors for a variety of microorganisms and cell lines.
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Lab products found in correlation
Soy peptone, by Biolife (1 mentions)
Di potassium hydrogen phosphate trihydrate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Honeywell (1 mentions)
Cary 670 ftir spectrometer, by Agilent Technologies (1 mentions)
Uv 1600 pc series spectrophotometer, by Avantor (1 mentions)
D glucose, by Merck Group (1 mentions)
Histidine, by Merck Group (1 mentions)
Tween 80, by Biolife (1 mentions)
Yeast extract, by Biolife (1 mentions)
2 protocols using tryptone
1
Spectroscopic Analysis of Organic Compounds
2
Histidine Decarboxylase Detection Protocol
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The culture was tested for histidine decarboxylase production according to Joosten and Northolt (16 (link)). Medium used for detection of decarboxylating bacteria contained (in %): 0.5 tryptone (Biolife), 0.5 yeast extract (Biolife), 0.5 NaCl (Gram-Mol) 0.1 glucose (Gram-Mol), 0.05 Tween 80 (Biolife), 0.02 MgSO4·7H2O (Kemika, Zagreb, Croatia), 0.01 CaCO3 (Kemika), 0.006 bromocresol purple (Kemika), 0.005 MnSO4·4H2O (Kemika), 0.004 FeSO4·7H2O (Kemika), 2 agar (Biolife), and 2 histidine (Sigma-Aldrich, Merck). After sterilisation (at 121°C for 10 min), the pH was 5.0±0.1. Cells of L. plantarum O1 overnight culture were centrifuged (Z206A; Hermle) at 8000×g for 10 min, washed twice with distilled water and resuspended in 0.5 mL of the same solvent. A volume of 0.1 mL of thus prepared suspension was inoculated in the middle of the plate (without spreading) and plates were incubated anaerobically at 37 °C for five days. Test was positive for amine production if the fully grown colonies were surrounded by a purple halo.
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