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Anti p40

Manufactured by Merck Group
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Anti-p40 is a laboratory reagent used for the detection and quantification of the p40 protein in biological samples. It serves as a tool for researchers and scientists to analyze the presence and levels of the p40 protein, which is a subunit of the interleukin-12 (IL-12) and interleukin-23 (IL-23) cytokines.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (2 mentions) Anti p47, by Santa Cruz Biotechnology (2 mentions) Anti neutrophil elastase, by Abcam (2 mentions) Anti human mouse myeloperoxidase mpo, by R&D Systems (2 mentions) Anti c albicans, by Merck Group (2 mentions) Anti cd63, by Merck Group (2 mentions) Anti histone h3 citrulline r2 r8 r17, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (2 mentions)

2 protocols using anti p40

1

Neutrophil Immunofluorescence Staining

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5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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2

Neutrophil Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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