Hp 5 m gc column

Aliquots (10%) of the Pronase glycopeptide fractions were dried and mixed with 1 nmol scyllo-inositol internal standard and subjected to methanolysis, re-N-acetylation and trimethylsilylation and GC-MS monosaccharide composition analysis of the resulting 1-O-methyl-glycoside TMS derivatives, as described in (50 ). Remaining 90% of the samples were used for methylation linkage analysis (50 ). Briefly, the samples were dried and subjected to permethylation using the sodium hydroxide method. The permethylated glycans were then subjected to acid hydrolysis, NaB(²H)₄ reduction, and acetylation to generate partially methylated alditol acetates (PMAAs). The 1-O-methyl-glycoside TMS derivatives and the PMAAs were analyzed by GC-MS (Agilent Technologies, 7890B Gas Chromatography system with 5977A MSD, equipped with Agilent HP-5 m GC Column, 30 m × 0.25 mm, 0.25 μm) as described in (50 ).