Anti aβ

Most general reagents used in this study were sourced from Sigma-Aldrich. The siRNA oligonucleotides were purchased from Dharmacon. Primary antibodies used in this study were as follows: anti-TBC1D5 [Santa Cruz, sc-376296, dilution 1:400 or 1:1000 for immunofluorescence (IF) microscopy or western blotting (WB), respectively], anti-VPS26 (Abcam, ab23892, 1:800 IF or 1:1000 WB), anti-VPS35 [Santa Cruz, sc-374372, 1:800 IF or 1:1000 WB, or from the Seaman lab (see Seaman, 2007 (link)), 1:300 for IF], anti-CIMPR (Abcam, ab2733, 1:400 IF or 1:1000 WB), anti-Lamp1 (Santa Cruz, sc-18821, 1:500 IF or 1:1000 WB), anti-Glut1 (Abcam, ab15309, 1:400 IF), anti-GM130 (BD Transduction labs 610822, 1:500 IF), anti-Fam21 (Millipore, ABT79, 1:400 IF or 1:1000 WB), anti-Aβ (Covance, SIG-39320, 1:1000 WB), anti-sAPPβ (IBL America, 10321, 1:800 WB), anti-Rab7a:GTP (NewEast Biosciences, 26923, 1:300 IF), anti-TGN46 (Seaman lab, see Seaman, 2007 (link), dilution 1:600 IF), anti-GFP (Seaman lab, see Seaman et al., 2009 (link), 1:1000 for immunoprecipitation), anti-Snx1 (BD Transduction labs, dilution 1:400 IF or 1:1000 WB) and anti-Tubulin (Sigma-Aldrich, dilution 1:1000 WB). Secondary fluorescently labelled antibodies were purchased from Invitrogen.

DL-2-amino-5-phosphonopentanoic acid (AP5), ifenprodil, 30% hydrogen peroxide (H₂O₂), piceid, forskolin, Hexafluoro-2-propanol (HFIP), glutaraldehyde, Trichloroacetic Acid (TCA) and human recombinant insulin were purchased from Sigma (St Louis, MO). Anti-MAP2 was from Sigma (St Louis, MO) Anti-APP (1G6) mouse monoclonal antibody raised against amino acid 573-596 of APP was purchased from Axxora; anti-APP (22C11) mouse monoclonal antibody against N terminus APP was developed in our laboratory; anti-Aβ (N terminus amino acid 1-12) mouse monoclonal antibody B436 was a gift from Dr. Steve L. Wagner (TorreyPines Therapeutics, Inc.); anti-Aβ (amino acid residues 17–24) mouse monoclonal antibody 4G8 was purchased from Covance. Anti-phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7), anti-IRβ, anti-phospho-CREB (Ser133), and anti-CREB antibodies were from Cell Signaling Technology (Danvers, MA).