Hacat

MDA-MB-231 (Sigma Aldrich, Vienna, Austria), HaCat (ATCC, US), MCF10A (LGC Promochem, US), A375 (CLS, Germany), SW-872 (ATCC, US), 93T449 (ATCC, US), SW1353 (CLS, Germany) and juvenile fibroblasts fresh established from foreskin samples were obtained from the Center of Medical Research (ZMF), Medical University of Graz, Austria. HeLa (ATCC ® , Guernsey, UK), fibroblast, HaCat, SW1353 and A375 cells were cultured in DMEM supplemented with 2 mM glutamine, 1% PS (100 U/mL penicillin, 100 µg/mL streptomycin) and 10% fetal bovine serum (FBS). MCF10A were cultured in DMEM with single quot kit suppl. Gr, 5% Horse Serum, 20 ng/mL hEGF, 0.5 µg/ml hydrocortison, 100 ng/ml choleratoxin, 10 µg/ml insulin and 2 mM glutamine. MDA-MB-231 were maintained in DMEM Hams F12 with 10% FBS, 2 mM glutamine and 1% PS. SW872 were cultured in DMEM Hams F12 supplemented with 5% FBS, 2 mM glutamine and 1% PS. 93T449 were cultured with RPMI-1640 with 10% FBS, 2 mM glutamine, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1mM sodium pyruvate and 1% PS.
Cells were maintained in a humidified incubator at 37°C with 5% CO2. HeLa cells were treated for up to 3 days with AdOx (40 µM), MS023 (10 µM), GSK715 (2 µM), GSK591 (1 µM) and DMSO, before cell extracts were prepared.