Cholestest cho

Manufactured by Sekisui

Sourced in Japan

Cholestest CHO is a lab equipment product designed to measure cholesterol levels. It provides a quantitative analysis of total cholesterol in a sample.

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Lab products found in correlation

Cholestest tg, by Sekisui (19 mentions) Cholestest ldl, by Sekisui (4 mentions) Cholestest n hdl, by Sekisui (4 mentions) Cholescolor liquidfc, by Toyobo (3 mentions) Multi bead shocker, by Yasui Kikai (2 mentions) Pureauto s tg n, by Sekisui (1 mentions)

9 protocols using cholestest cho

Lipid Extraction and Quantification

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Liver tissues were homogenized in chloroform/methanol (2:1, v/v), and lipid extracts were prepared using the Folch method^{35 (link)}. Intrahepatic cholesterol and TG levels were measured using Cholestest CHO and Cholestest TG (Sekisui Medical, Tokyo, Japan), respectively.

Kawano Y., Tanaka M., Satoh Y., Sugino S., Suzuki J., Fujishima M., Okumura E., Takekoshi H., Uehara O., Sugita S., Abiko Y., Tomonari T., Tanaka H., Takeda H, & Takayama T. (2024). Acanthopanax senticosus ameliorates steatohepatitis through HNF4 alpha pathway activation in mice. Scientific Reports, 14, 110.

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Cholesterol Measurement by Homogeneous Assay

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The cholesterol levels were measured by a homogeneous assay, Cholestest LDL (SEKISUI Medical, Tokyo, Japan) for LDL-C, CholestestN HDL (SEKISUI Medical) for HDL-C and Cholestest CHO (SEKISUI Medical) for TC. Non-HDL-C was calculated by subtracting HDL-C from TC.

Komatsu T., Miura T., Joko K., Sunohara D., Mochidome T., Kasai T, & Ikeda U. (2021). Real-world Profile of a Selective Peroxisome Proliferator-activated Receptor α Modulator (SPPARMα) in Japanese Patients with Renal Impairment and Dyslipidemia. Internal Medicine, 60(17), 2741-2748.

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Hepatic Lipid Extraction and Quantification

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Hepatic lipids were extracted according to a previous method [14 (link)]. Briefly, 100 mg of frozen hepatic pieces were homogenized in 2 mL of cooled chloroform-methanol solution (2:1) using a multibead shocker (Yasui Kikai Corporation, Osaka, Japan). Filtered samples were adjusted to 4 mL with chloroform-methanol solution and were washed with 0.8 mL of purified water. Subsequent washes were performed by adding 3.75 mL of chloroform-methanol-water solution (2:1:0.75), and the resulting extracts were dried by evaporation. Extracted lipids were resolved with 1 mL of isopropanol.
Hepatic TG, total cholesterol, and total bile acids were measured using Cholestest TG, Cholestest CHO (Sekisui Medical, Tokyo, Japan), and total bile acids assay kits (Diazyme Laboratories, Poway, CA, USA), respectively.

Tanaka M., Yasuoka A., Shimizu M., Saito Y., Kumakura K., Asakura T, & Nagai T. (2017). Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats. Genes & Nutrition, 12, 10.

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Quantification of Intrahepatic Lipids and Bile Acids

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Quantification of intrahepatic lipids and total bile acid (TBA) was conducted at Skylight Biotech, Inc. (Akita, Japan). Lipids and TBA were extracted by the Folch method and ethanol, respectively [29 (link)]. Triglyceride (TG), total cholesterol (T-CHO), free cholesterol (F-CHO), and TBA concentrations were quantified using the Cholestest^® TG, Cholestest^® CHO (Sekisui Medical, Tokyo, Japan), Cholescolor Liquid FC (Toyobo, Osaka, Japan), and Total Bile Acids Assay Kit (Diazyme Laboratories, Inc., Poway, CA, USA), respectively. The TG, T-CHO, F-CHO, and TBA levels are presented as mg/g, and were calculated by dividing the amount of detected analyte by the weight of liver tissue.

Okushin K., Tsutsumi T., Ikeuchi K., Kado A., Enooku K., Fujinaga H., Yamauchi N., Ushiku T., Moriya K., Yotsuyanagi H, & Koike K. (2020). Heterozygous knockout of Bile salt export pump ameliorates liver steatosis in mice fed a high-fat diet. PLoS ONE, 15(8), e0234750.

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Hepatic Lipid Content Analysis

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Hepatic lipid content was analyzed by Skylight Biotech (Akita, Japan). Liver tissues were homogenized in chloroform/methanol (2:1, v/v), lipid extracts were prepared using the Folch method (Folch et al., 1957 (link)) and intrahepatic TC, TG and PL levels were measured using the enzymatic assay kits, Cholestest CHO, Cholestest TG and Pureauto S PL (Sekisui Medical, Tokyo, Japan), respectively.

Owaki T., Kamimura K., Ko M., Nagayama I., Nagoya T., Shibata O., Oda C., Morita S., Kimura A., Sato T., Setsu T., Sakamaki A., Kamimura H., Yokoo T, & Terai S. (2022). Involvement of the liver-gut peripheral neural axis in nonalcoholic fatty liver disease pathologies via hepatic HTR2A. Disease Models & Mechanisms, 15(7), dmm049612.

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Analyzing Hepatic Lipid Levels

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Determination of TG and CHO levels in liver samples was entrusted to Skylight Biotech, Inc. (Akita, Japan). Liver tissues were homogenized in chloroform/methanol (2:1, v/v), and lipid extracts were prepared using the Folch method (Folch et al., 1957 (link)). Intrahepatic TG and CHO levels were measured using enzymatic assay kits (Cholestest®TG and Cholestest®CHO, respectively; Sekisui Medical Co., Ltd., Tokyo, Japan).

Tometsuka C., Funato N., Mizuno K, & Taga Y. (2021). Long-term intake of ginger protease-degraded collagen hydrolysate reduces blood lipid levels and adipocyte size in mice. Current Research in Food Science, 4, 175-181.

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Hepatic Lipid Composition Analysis

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Determination of TAG as well as total and free cholesterol levels in liver samples from all animals was entrusted to Skylight Biotech, Inc. The lipid components of the liver samples were extracted using chloroform-methanol mixture solution, and determined by absorbance measurements based on enzymatic methods (reagent kits: Cholestest ® TG or Cholestest ® CHO; Sekisui Medical Co., Ltd and Cholescolor ® Liquid FC; Toyobo Co., Ltd).

Tometsuka C., Koyama Y.I., Ishijima T., Toyoda T., Teranishi M., Takehana K., Abe K, & Nakai Y. (2017). Collagen peptide ingestion alters lipid metabolism-related gene expression and the unfolded protein response in mouse liver. The British journal of nutrition, 117(1).

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Maternal Metabolic Markers During Pregnancy

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Self‐administered questionnaires were carried out during early and late pregnancy, as described previously20.
The blood of participants was collected around 12 and 32 weeks of gestation. The blood was separated into serum and stored at −80°C in Chiba University Center for Preventive Medical Sciences BioBank before the measurement. The maternal serum levels of glycoalbumin, total cholesterol, and triglycerides during early and late pregnancy were measured using Lucica GA‐L (Asahi Kasei Pharma Corporation, Tokyo, Japan), Cholestest CHO (Sekisui Medical Co., Ltd., Tokyo, Japan) and Pureauto S TG–N (Sekisui Medical Co., Ltd.), respectively, at SRL (SRL, Inc., Shinjuku, Tokyo, Japan).

Sakurai K., Kato T., Tanabe H., Taguchi‐Atarashi N., Sato Y., Eguchi A., Watanabe M., Ohno H, & Mori C. (2019). Association between gut microbiota composition and glycoalbumin level during pregnancy in Japanese women: Pilot study from Chiba Study of Mother and Child Health. Journal of Diabetes Investigation, 11(3), 699-706.

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Serum Lipid Profiling Protocol

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Brunt's classification [21] at Sapporo City General Hospital. Patients who met any of the following criteria were excluded in this study: alcohol abuse (≥20 g/day), hepatitis B and hepatitis C virus infection, autoimmune liver disease, primary biliary cholangitis and Wilson's disease. Sera were obtained by centrifugation (2,000 x g, 10 min, room temperature). Serum lipid levels were measured by automated enzymatic methods using commercial kits (Sekisui Medical Co., Ltd., Tokyo, Japan): Cholestest CHO for total cholesterol (TC), Cholestest TG for triglyceride, Pureauto S PL for phospholipid (PL), Qualigent LDL for LDL-cholesterol, and Qualigent HDL for HDL-cholesterol.

Ikuta A., Sakurai T., Nishimukai M., Takahashi Y., Nagasaka A., Hui S.P., Hara H, & Chiba H. (2019). Composition of plasmalogens in serum lipoproteins from patients with non-alcoholic steatohepatitis and their susceptibility to oxidation. Clinica chimica acta; international journal of clinical chemistry, 493.

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