Cells were lysed in ice cold RIPA buffer, incubated for 30 min in 4 °C and centrifuged at 12,500× g rpm for 20 min at 4 °C. Samples were resolved using SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (PVDF) (Thermo Fisher). GR protein was detected with monoclonal anti-Glucocorticoid Receptor antibody (ab183127, Abcam, Cambridge, UK), and secondary anti-rabbit antibody conjugated to HRP (#7074, Cell Signaling, Beverly, MA, USA). Glyceraldehyde-3-Phosphate Dehydrogenase (#MAB374, Millipore, Bedford, MA, USA) detected with mouse HRP-conjugated antibody (#7076 Cell Signaling) served as control. Visualization was performed with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) and CCD digital imaging system Alliance Mini HD4 (UVItec Limited, Cambridge, UK).
Alliance mini hd4
Manufactured by Uvitec
Sourced in United Kingdom
The Alliance Mini HD4 is a compact and versatile laboratory equipment designed for accurate and efficient analysis. It features a high-definition camera and advanced imaging capabilities to capture detailed images and video of samples. The device is suitable for a wide range of applications in various research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride (pvdf), by Thermo Fisher Scientific (3 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (3 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Merck Group (1 mentions)
Westernbright quantum, by Advansta (1 mentions)
Pa5 16777, by Thermo Fisher Scientific (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase, by Merck Group (1 mentions)
Lamin a c antibody, by Santa Cruz Biotechnology (1 mentions)
4 protocols using alliance mini hd4
1
Western Blot Analysis of Glucocorticoid Receptor
2
Western Blot Analysis of DLC1 Protein
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Cells were lysed in ice cold RIPA buffer, incubated for 30 min in 4 °C and centrifuged at 12,500 rpm for 20 min at 4 °C. Samples were resolved using SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (PVDF) (Pierce/Thermo Fisher). DLC1 was detected with polyclonal anti-DLC1 antibody (PA5-28443, Pierce/Thermo Fisher), and secondary anti-rabbit antibody conjugated to HRP (#7074, Cell Signalling). α-tubulin detected with polyclonal rabbit antibody (#2125, Cell Signalling) or β-actin detected with mouse HRP-conjugated antibody (#12262 Cell Signalling) served as control. Visualization was performed with the enhanced chemiluminescence method using WesternBright Quantum (Advansta, Menlo Park, CA, USA) and CCD digital imaging system Alliance Mini HD4 (UVItec Limited, Cambridge, United Kingdom).
3
Western Blot Analysis of Cyclin D1
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Cells were lysed in ice-cold RIPA buffer, incubated for 30 min at 4 °C, and centrifuged at 12,500 rpm at 4 °C for 20 min. Samples were electrophoresed with SDS-PAGE and transferred to polyvinylidene fluoride membranes (PVDF) (Thermo Fisher Scientific, Waltham, MA, USA). Cyclin D1 was detected with rabbit polyclonal antibody (PA5-16777, Thermo Fisher Scientific) and secondary anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (#7074, Cell Signalling). Anti-lamin A + C antibody (ab108922, Abcam, Cambridge, England) detected with rabbit HRP-conjugated antibody served as the control. SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) and CCD digital imaging system Alliance Mini HD4 (UVItec Limited, Cambridge, United Kingdom) were used for visualization. ImageJ 1.52i software (National Institutes of Health, Bethesda, MD, USA) was used for densitometry.
4
Western Blot Analysis of USP8 and p27
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Cells were lysed in ice cold RIPA buffer, incubated for 30 min in 4 °C and centrifuged at 14,400× g for 20 min at 4 °C. Samples were resolved using SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (PVDF) (Thermo Fisher Scientific). USP8 protein was detected with USP8 Rabbit polyclonal antibody (27791-1-AP, Proteintech, Rosemont, IL, USA), p27 protein was detected with Anti-p27 KIP1 antibody (ab32034, Abcam, Waltham, MA, USA). Reference proteins Lamin A/C and Glyceraldehyde-3-Phosphate Dehydrogenase were detected with Lamin A/C antibody (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA) and Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (#MAB374, Sigma-Aldrich), respectively. Anti-rabbit antibody conjugated to HRP (#7074, Cell Signalling, Danvers, MA, USA) was used as secondary antibody. SuperSignal West Pico Chemiluminescent Substrate (no. 34087, Thermo Fisher Scientific) and the CCD digital imaging system Alliance Mini HD4 (UVItec Limited, Cambridge, United Kingdom) were used for visualization. Densitometry was analyzed with ImageJ software (National Institutes of Health, Bethesda, MD, USA).
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