The HaCat line is the keratinocyte cell from human skin and purchased from the American Type Culture Collection (ATCC, Rockville, MD). In our research, we used this cell line to study the subcellular location of Foxp1 protein. Due to the low transfection efficiency of HaCat cells, we used HEK293T cells to perform western blot. The CHO cell line is the Chinese hamster ovary cells. In order to examine the modification of mouse Foxp1 protein under oxidative stress, we choose a mouse CHO cell line instead of human 293T cells. The HeLa cells that were tolerant to oxidative stress were used here is to test the role of Foxp1 in antagonizing Trx1 function. Besides, cells were cultured in high DMEM medium added with 10% FBS, 1% penicillin, and 1% streptomycin at 37°C and 5% CO2.
Hacat line
Manufactured by American Type Culture Collection
Sourced in United States
The HaCat line is a collection of immortalized human keratinocyte cell lines derived from normal human skin. These cell lines are widely used in cell biology research and offer a consistent, renewable source of human epithelial cells for a variety of applications.
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2 protocols using hacat line
1
Foxp1 Subcellular Localization and Oxidative Stress
2
Comparative Analysis of Cervical Cancer Cell Lines
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The cervical cancer CaSki, HeLa (HPV-18-positive), SiHa (HPV-16-positive) and C-33A (HPV-negative) cell lines were compared with the human keratinocyte cell HaCaT line and obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI-1640 (GE Healthcare Life Sciences, Little Chalfont, UK). All cultures were supplemented with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), streptomycin (100 mg/ml; GE Healthcare Life Sciences) and penicillin (100 IU/ml; GE Healthcare Life Sciences) at 37°C in a humidified atmosphere containing 5% CO2.
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