Dnasis ver 2

Gene encoding proteins, rRNA and tRNA were identified according to their amino acid translation or secondary structure features, respectively. Individual gene sequences were compared with the available homologous sequences of Orthoptera species in GenBank. A total of 22 tRNA genes were identified using software tRNA Scan-SE 1.21 (http://lowelab.ucsc.edu/tRNAscan-SE) and their cloverleaf secondary structures and anticodon sequences were identified using DNASIS (Ver.2.5, Hitachi Software Engineering).

Sequences were checked and assembled using the programs Seqman (DNASTAR, 2001), BioEdit, and Chromas 2.22, and then adjusted manually. PCGs were identified by comparison with the known complete mtDNA sequences of Anseriform birds using Sequin 11.0. The 22 tRNA genes were identified using the software package tRNA Scan-SE 1.21 (http://lowelab.ucsc.edu/tRNAscan-SE), and their cloverleaf secondary structures and anticodon sequences were determined using DNASIS (Ver.2.5, Hitachi Software Engineering). Two rRNAs were identified by comparison with complete mtDNA sequences of other Anseriformes available in GenBank. The complete mtDNA sequences have been deposited in GenBank under accession numbers KF437906 and KF684946.