Immunoblotting was carried out as described previously43 (link). Briefly, cells were washed 3 times with PBS and lysed in ice-cold RIPA lysis buffer supplemented with protease inhibitor (Roche) for 5 minutes. Immunoblotting was performed with the following antibodies: p53 (Santa Cruz, DO-1, SC126), p-p53 Ser-15 (Cell Signaling,9284), total PARP and cleaved PARP (Cell Signaling, 9542 and 5625), caspase 3 and cleaved caspase 3 (Cell Signaling, 9664 and 9665), Beta Actin (Sigma), p21 (Cell Signaling,2947).
Total parp and cleaved parp
Manufactured by Cell Signaling Technology
Total PARP and cleaved PARP are lab equipment products manufactured by Cell Signaling Technology. They are used for the detection and quantification of the PARP protein and its cleaved form. The products provide a reliable and reproducible method for measuring these proteins in biological samples.
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2 protocols using total parp and cleaved parp
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Immunoblotting Procedure for Protein Analysis
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Immunoblotting Procedure for Protein Analysis
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Immunoblotting was carried out as described previously43 (link). Briefly, cells were washed 3 times with PBS and lysed in ice-cold RIPA lysis buffer supplemented with protease inhibitor (Roche) for 5 minutes. Immunoblotting was performed with the following antibodies: p53 (Santa Cruz, DO-1, SC126), p-p53 Ser-15 (Cell Signaling,9284), total PARP and cleaved PARP (Cell Signaling, 9542 and 5625), caspase 3 and cleaved caspase 3 (Cell Signaling, 9664 and 9665), Beta Actin (Sigma), p21 (Cell Signaling,2947).
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