Nmri nu female mice

Cultured cells were harvested by trypsinization, washed and resuspended in saline buffer. Cell suspensions were injected s.c. as 10⁶ cells in 0.2 ml volume in the flank of 7-/8-week-old nude NMRI-nu female mice (Janvier, France).
The experimental procedures using animals were in accordance with the guidelines of Institutional Animal Care and the French National Committee of Ethics. In addition, all experiments were conducted according to the Regulations for Animal Experimentation at the « Plate-forme Animalerie » of « Institut de Recherche en Santé de l'Université de Nantes (IRS-UN) » and approved by the French National Committee of Ethics (Aggrement number: B44278).

After diuron exposure, Ntv-a cells were harvested by trypsinization, washed, and resuspended in saline buffer. Cell suspensions were injected s.c. (subcutaneous) as 2.10⁶ cells in 0.05 ml of PBS with equal volume of matrigel matrix (Becton Dickinson, France) in the flank of seven groups of five 7/8-week-old nude NMRI-nu female mice (Janvier, France). Tumor volumes based on caliper measurements were calculated by the modified ellipsoidal formula (tumor volume = 1/2 (length × width²)).
The experimental procedures using animals were in accordance with the guidelines of Institutional Animal Care and the French National Committee of Ethics. In addition, all experiments were conducted according to the Regulations for Animal Experimentation at the “Plate-forme Animalerie” of “Institut de Recherche en Santé de l’Université de Nantes (IRS-UN)” and approved by the French National Committee of Ethics (Agreement number: B44278).