Laborimpex

Manufactured by Agar Scientific

Laborimpex is a laboratory equipment manufacturer. Their product line includes a range of devices and instruments used in scientific research and analysis. Without further interpretation, the core function of Laborimpex products is to provide high-quality laboratory equipment for various applications.

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Lab products found in correlation

Jem 1400, by JEOL (2 mentions) Item 5, by Olympus (2 mentions) Em uc7, by Leica (1 mentions) Quemesa, by Olympus (1 mentions) Quemasa, by Olympus (1 mentions)

3 protocols using laborimpex

Ultrastructural Characterization of Synaptic Vesicles

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For TEM, larvae were dissected in HL-3 and fixed immediately in 1% glutaraldehyde, 4% paraformaldehyde, and 1 mM MgCl₂ in 0.1 M Na-cacodylate buffer, pH 7.4. Subsequently, specimens were osmicated in OsO₄/Na-cacodylate buffer for 2 h and stained in 2% aqueous uranyl acetate (1.5 h). After dehydration using a series of ethanol, specimens were embedded in Agar 100 (Laborimpex; Agar Scientific). Ultrathin sections (60–70 nm) were cut with an ultramicrotome (EM UC7; Leica), collected on grids (Laborimpex; Agar Scientific), and coated with Butvar. Sections were imaged using a transmission electron microscope (JEM 1400; JEOL) at 80 kV. Micrographs were acquired using a bottom-mounted camera (Quemesa; 11 megapixels; Olympus) using iTEM 5.2 software (Olympus). Ultrastructural features were quantified with ImageJ from boutonic profiles whose surface area is ≥1 µm². Diameters of synaptic vesicles were measured from profiles with clearly visible membranes. EM micrographs were processed in Photoshop using brightness and contrast.

Fernandes A.C., Uytterhoeven V., Kuenen S., Wang Y.C., Slabbaert J.R., Swerts J., Kasprowicz J., Aerts S, & Verstreken P. (2014). Reduced synaptic vesicle protein degradation at lysosomes curbs TBC1D24/sky-induced neurodegeneration. The Journal of Cell Biology, 207(4), 453-462.

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Ultrastructural Analysis of Adult Fly Retina

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Adult fly heads were dissected and immediately fixed in 4% paraformaldehyde and 2% glutaraldehyde in 0.1 M Na-Cacodylate buffer (pH 7.4) for 2 h at room temperature. Samples were further fixed at 4 °C overnight, and then washed with 0.1 M Na-Cacodylate, pH 7.4, and subsequently osmicated with 2% osmium (OsO₄/Na-Cacodylate buffer). After dehydration in an ascending series of ethanol solutions and staining in 4% aqueous uranyl acetate solution the specimen were embedded in Agar 100 (Laborimpex; Agar Scientific). Ultrathin sections (70 nm) of the retina and the lamina were collected on grids (Laborimpex; Agar Scientific) coated with Butvar and imaged on a JEM 1400 transmission electron microscope (JEOL) at 80 kV with a bottom mounted camera (Quemasa; 11 megapixels; Olympus) running iTEM 5.2 software (Olympus).

Zhou L., McInnes J., Wierda K., Holt M., Herrmann A.G., Jackson R.J., Wang Y.C., Swerts J., Beyens J., Miskiewicz K., Vilain S., Dewachter I., Moechars D., De Strooper B., Spires-Jones T.L., De Wit J, & Verstreken P. (2017). Tau association with synaptic vesicles causes presynaptic dysfunction. Nature Communications, 8, 15295.

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Ultrastructural Analysis of Neuromuscular Synapses

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Third instar larvae were dissected in HL-3 and motor neurons cut. Preparations were stimulated for 1 min in HL-3 with KCl (60 mM KCl and 1.5 mM CaCl 2 ), followed by fixation (1% glutaraldehyde, 4% paraformaldehyde, 1 mM MgCl 2 in 0.1 M Na-cacodylate buffer, pH 7.4).
Subsequently, larvae were osmicated in OsO 4 /Na-cacodylate for 2 h and stained in 2% aqueous uranyl acetate for 1.5 h. Specimens were dehydrated in a graded ethanol series and embedded in Agar 100 (Laborimpex, Agar Scientific). Ultrathin sections (60-70 nm) were collected on butvar-coated grids (Laborimpex, Agar Scientific). Grids were recorded with a JEM1400 (JEOL)
transmission electron microscope at 80 kV. To analyze synaptic boutons, ultrastructural profile areas and synaptic vesicle content were quantified in ImageJ (NIH) as single-blinded data.

Fischer B., Lüthy K., Paesmans J., De Koninck C., Maes I., Swerts J., Kuenen S., Uytterhoeven V., Verstreken P, & Versées W. (2016). Skywalker-TBC1D24 has a lipid-binding pocket mutated in epilepsy and required for synaptic function. Nature structural & molecular biology, 23(11).

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