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2 protocols using 2 chloronaphtahalene

1

Metabolite Profiling Protocol

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First grade methanol, hexane, water, D2O [99.%, containing 0.05% 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt (TSP) as internal standard for NMR], methoxylamine hydrochloride, pyridine, L-ascorbic acid, 1,1-diphenyl-2-picrylhydrzyl (DPPH), Folin-Ciocalteu's phenol reagent, gallic acid, and sodium carbonate were purchased from Sigma (St. Louis, MO, USA). NaOD were obtained from Cortec (Paris, France). BSTFA [N,O-bis (trimethylsilyl) trifluoroacetamide containing 1% TMCS (trimethyl chlorosilane)] were purchased from Alfa Aesar (Ward Hill, MA, USA) and 2-Chloronaphtahalene as internal standard for GC-MS were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan).
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2

Analytical Reagents for Phytochemical Analysis

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HPLC-grade methanol, water, Murashige and Skoog (MS) powder, pyridine, 1,1-diphenyl-2-picrylhydrazyl (DPPH), ascorbic acid, dimethyl sulfoxide (DMSO), Folin-Ciocalteu’s reagent, sodium carbonate, gallic acid, sodium nitrite, aluminum chloride, Catechin, and methoxyamine were purchased from Sigma (St. Louis, MO, USA). BSTFA [N,O-bis(trimethylsilyl)trifluoroacetamide containing 1% trimethyl chlorosilane (TMCS)] was obtained from Alfa Aesar (Ward Hill, MA, USA), and 2-Chloronaphtahalene (internal standard for GC-MS) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
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