Cct020312

Dexmedetomidine (Heng Rui Pharmaceutical Co. Ltd., Lianyungang, Jiangsu, China); Trizol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA); RIPA lysis, phenylmethanesulfonyl fluoride (PMSF), and phosphate buffered saline (PBS) (Beyotime, Shanghai, China); 2,3,5-triphenyltetrazolium chloride (TTC) and dimethyl sulfoxide (DMSO) (Sigma, St. Louis, MO, USA); DMEM (HyClone, Logan, UT, USA); FBS (Biological Industries, Kibbutz Beit-Haemek, Israel); glucose-free DMEM (Gibco, Carlsbad, CA, USA); reverse transcription polymerase chain reaction (RT-PCR) kit (Applied Biological Materials, Richmond, BC, Canada); RT-PCR primers (Sangon, Shanghai, China); anti-GRP78, anti-CHOP, and anti-cleaved caspase 3 (Cell Signaling Technology, Beverly, MA, USA); anti-α2A-AR (Santa Cruz, Dallas, TX, USA); anti-IRE1, anti-phosphorylated-IRE1 (anti-p-IRE1), anti-ATF6, anti-α2B-AR, and anti-caspase 3 (abcam, Cambridge, MA, USA); anti-phosphorylated-PERK (anti-p-PERK) and anti-PERK (ABclonal, Wuhan, Hubei, China); anti-GAPDH (Absin, Shanghai, China); yohimbine (Selleck, Houston, TX, USA); CCT020312 (MedChemExpress, Monmouth Junction, NJ, USA).

PC12 rat adrenal pheochromocytoma cells were obtained from Icell Bioscience Inc., Shanghai. BUP hydrochloride and RSV were purchased from Sigma-Aldrich (Merck KGaA), while CCT020312 and EX527 were obtained from MedChemExpress. High-glucose Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific, Inc.). The Cell Counting Kit-8 (CCK-8) was supplied by Biosharp Life Sciences, and the Annexin V/7-aminoactinomycin D (7-AAD) apoptosis detection kit was provided by BD Biosciences. Antibodies against SIRT1 (cat. no. ab110304) and caspase-12 (cat. no. ab62484) were purchased from Abcam; antibodies against PERK (cat. no. 3192S), phosphorylated (p)-eIF2α (cat. no. 3398T), eIF2α (cat. no. 5324T), CHOP (cat. no. 5554T) and ATF4 (cat. no. 11815) were supplied by Cell Signaling Technology, Inc.; antibodies against Bax (cat. no. 60267-1-Ig), Bcl-2 (cat. no. 26593-1-AP), glucose-regulated protein 78 (GRP78; cat. no. 11587-1-AP) and GAPDH (cat. no 10494-1-AP) were provided by Proteintech Group, Inc.; and antibodies against cleaved caspase-3 (cat. no. WL02117) and p-PERK (cat. no. WL05295) were obtained from Wanleibio Co., Ltd.