Biophotometer plus uv vis equipment

Manufactured by Eppendorf

Sourced in United States

The BioPhotometer Plus is a UV-vis spectrophotometer designed for accurate measurement of sample absorbance. It features a wavelength range of 200 to 1,100 nm and supports a variety of cuvette formats. The instrument provides precise quantification of nucleic acids, proteins, and other biomolecules.

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Lab products found in correlation

Bradford protein assay, by Bio-Rad (2 mentions) Pd 10, by GE Healthcare (2 mentions)

Spelling variants of this product

BioPhotometer (764 citations) BioPhotometer Plus (385 citations) UV-VIS Biophotometer (5 citations) UV BioPhotometer (3 citations)

2 protocols using biophotometer plus uv vis equipment

Purification of Chia Enzymes from Stomach Tissue

Cited 2 times

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The Chia enzymes were purified from chicken glandular stomach or pig stomach tissue using a chitin bead column and eluted with 8 M urea, as described previously [16 (link),17 (link)].
The enzymes were also eluted from the columns with 0.1 M acetic acid. The eluted enzymes were neutralized and desalted with PD10 (GE Healthcare, Piscataway, NJ, USA) equilibrated by TS buffer.
Protein concentrations were determined by the Bradford Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA) using BioPhotometer Plus UV-vis equipment (Eppendorf, Hamburg, Germany). Bovine serum albumin was used as the standard.

Tabata E., Kashimura A., Wakita S., Sakaguchi M., Sugahara Y., Imamura Y., Shimizu H., Matoska V., Bauer P.O, & Oyama F. (2018). Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes. International Journal of Molecular Sciences, 19(2), 362.

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Purification of Natural Chia Enzyme

Cited 1 time

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The natural Chia enzyme was purified from porcine stomach tissue using chitin beads column and eluted with 0.1 M acetic acid as described previously^{13 (link)}. The eluted enzymes were neutralized and desalted with PD10 (GE Healthcare) equilibrated by TS buffer.
Protein concentrations were determined by the Bradford Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA) using BioPhotometer Plus UV/Vis equipment (Eppendorf, Hamburg, Germany). Bovine serum albumin was used as the standard.

Tabata E., Wakita S., Kashimura A., Sugahara Y., Matoska V., Bauer P.O, & Oyama F. (2019). Residues of acidic chitinase cause chitinolytic activity degrading chitosan in porcine pepsin preparations. Scientific Reports, 9, 15609.

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