Transwell chambers

Manufactured by Wuhan Servicebio Technology

Transwell chambers are a type of cell culture system that allows for the physical separation of two cell populations while still enabling communication between them. The chambers consist of an upper and lower compartment divided by a porous membrane. This setup facilitates the study of cell migration, invasion, and permeability across the membrane.

Automatically generated - may contain errors

Lab products found in correlation

Paraformaldehyde (pfa), by Wuhan Servicebio Technology (1 mentions) Crystal violet, by Wuhan Servicebio Technology (1 mentions)

2 protocols using transwell chambers

Transwell Assay for CRC Cell Migration

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The migration and invasion abilities of CRC cells were evaluated by transwell assay. Each well above the transwell chambers (Servicebio) was inoculated with 5×10
⁴ cells and serum-free DMEM was added, and 10% fetal bovine serum was added below the chambers. The cells were incubated for 24 h, fixed in 4% paraformaldehyde (Servicebio) and stained with 0.1% crystal violet (Servicebio).

Wu F., Zhang W., Wei H., Ma H., Leng G, & Zhang Y. (2023). lncRNA ELFN1-AS1 promotes proliferation, migration and invasion and suppresses apoptosis in colorectal cancer cells by enhancing G6PD activity : lncRNA ELFN1-AS1 promotes the progression of colorectal cancer. Acta Biochimica et Biophysica Sinica, 55(4), 649-660.

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Transwell Cell Invasion Assay

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Cell invasion capacity was evaluated using Transwell chambers with 8 μm pore size (Servicebio, Wuhan, China). The bottom of the migration chamber was initially coated with Matrigel (37 °C; incubated for 3 h). Cultured in a Transwell system, 200 µL of the serum-free medium was added to the upper chamber, while 800 µL of medium supplemented with 20% FBS was added to the lower chamber. After 24 h of incubation, the cells were washed with PBS, fixed in 4% PFA, and stained with gentian violet. Following air-drying, the cells were photographed utilizing a microscope and counted.

Ke S., Guo J., Wang Q., Shao H., He M., Li T., Qiu T, & Guo J. (2023). Netrin Family Genes as Prognostic Markers and Therapeutic Targets for Clear Cell Renal Cell Carcinoma: Netrin-4 Acts through the Wnt/β-Catenin Signaling Pathway. Cancers, 15(10), 2816.

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