For anti-CD47 antibody injection, mice were injected i.t. with 50–70 µg anti-CD47 (clone MIAP301, BioXCell) 9 or 10 d and 13 or 14 d after tumor inoculation. For anti-CD47 antibody systemic injection, mice were injected i.p. with 500 µg anti-CD47 9 d and 13 d after tumor inoculation. For anti-IFNαR antibody injection, mice were injected i.t. with 200 µg anti-IFNαR antibody 6, 8, 10, 12, and 14 d after tumor inoculation. For DMXAA administration, mice were injected i.t. with 20 µg DMXAA either 9 or 13 d after tumor inoculation.
Anti cd47 clone miap301
Manufactured by BioXCell
Anti-CD47 (clone MIAP301) is a monoclonal antibody that targets the CD47 protein. CD47 is a cell surface protein involved in various cellular processes. The antibody is suitable for research applications, but its detailed function and intended use are not provided in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Mitotempo, by Thermo Fisher Scientific (1 mentions)
Ccf4 am, by Thermo Fisher Scientific (1 mentions)
Annexin 5 staining kit, by Cedarlane (1 mentions)
Mitosox, by Thermo Fisher Scientific (1 mentions)
Anti pd 1 clone rmp1 14, by BioXCell (1 mentions)
Rat igg2a isotype control clone 2a3, by BioXCell (1 mentions)
3 protocols using anti cd47 clone miap301
1
Immunotherapeutic Interventions in Murine Tumors
2
Immune Modulation with Antibodies
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Depletion antibodies (anti‐CD4, anti‐CD8, and anti‐NK1.1) as well as anti‐PD‐1 (clone RMP1‐14‐CP157), anti‐CTLA4 (clone 9D9), and anti‐CD47 (clone MIAP301) were purchased from BioXCell. The MitoSOX, CCF4‐AM, and MitoTEMPO reagents were purchased from Thermo Fisher Scientific and used according to the manufacturer's instructions. Cytochrome C (Cyt‐C), N‐acetylcysteine (NAC), and β‐lactamase were purchased from Millipore‐Sigma. The Annexin‐V staining kit was purchased from Cedarlane laboratories. The Accum™ and its leucine mutant derivative were synthesized as previously described.
15 (link)
15 (link)
3
Tumor Immunotherapy in Murine Models
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For studies with MC38 tumors, wild type C57BL/6 mice were implanted with MC38 cells as described above. When tumor volume reached an average of 75 mm3, mice were randomized into groups of n = 15 mice. For studies with E0771 tumors, Ptpn6fl/fl mice were implanted as described above. For anti-PD1 studies: mice were dosed intraperitoneally (IP) with anti-PD1 (clone RMP1-14, BioXCell) or rat IgG2a isotype control (clone 2A3, BioXCell) at 10 mg/kg twice weekly (BIW). For anti-CD47 studies, mice were dosed IP with anti-CD47 (clone miap301, BioXCell) or rat IgG2a isotype control (clone 2A3, BioXCell) at 20 mg/kg BIW for 2 weeks as described in Liu et al. (41 (link)).
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