Fm1 43

Manufactured by Abcam

Sourced in Australia

FM1-43 is a fluorescent dye used in cell and molecular biology research. It is a lipophilic styryl dye that can be used to study membrane dynamics and endocytosis in living cells. FM1-43 reversibly stains the outer leaflet of the plasma membrane, making it a useful tool for visualizing membrane trafficking processes.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (1 mentions) C11440 orca flash 4, by Hamamatsu Photonics (1 mentions) Bx51w1, by Olympus (1 mentions) Advasep 7, by Merck Group (1 mentions)

2 protocols using fm1 43

Visualizing GTP-induced GERV formation

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Microsomes were diluted in B88 buffer to a protein concentration of 0.5 mg ml⁻¹ as determined by a Bradford assay and mixed with the membrane dye FM1-43 (4-[2-[4-(dibutylamino)phenyl]ethenyl]-1-[3-(triethylammonio)propyl]pyridinium dibromide, available as MM1-43 from Abcam). The labeled microsomes were divided into 6 aliquots, mixed with 0, 2, 5, 10, 20, or 50 mM guanosine-5′-triphosphate (GTP) and incubated for 2 h. Images at different locations were taken on a Zeiss LSM 710 confocal microscope. For quantitation, GERVs which had a minimum diameter of 2 µm and at least one third of the membrane free of aggregates were taken into account. Data shown are from four different membrane preparations.

Grimmer M, & Bacia K. (2020). Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool. Scientific Reports, 10, 3100.

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Imaging Presynaptic Vesicle Release in Spinal Cord

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Lumbar spinal cord slices were incubated in Mg²⁺-free ACSF that contained (mM): 125 NaCl, 2.5 KCl, 1.25 NaH₂PO₄, 25 NaHCO₃, 2.5 CaCl₂, 10 glucose and 0.1 4-AP for 10 min to increase neuronal activity. Slices were transferred to Mg²⁺-free ACSF with 4-AP containing 8 μM FM1–43 (Abcam, Australia) for 3 min, washed with ACSF for 2 min, and then incubated in 1 mM ADVASEP-7 (Sigma) for 2 min. Slices were washed with ACSF for 2 min and the ADVASEP-7 incubation was repeated. Slices were placed in the recording chamber and washed for a further 15 min in ACSF prior to imaging. A bipolar stimulating electrode was placed in the dorsal root entry zone of the spinal cord and stimulated for 10 s at 1Hz, 4–6V to facilitate the release of vesicles from the presynaptic terminals. Optical recordings were completed on an upright fluorescence microscope (BX51W1, Olympus) under 40x magnification using a Cy3/TRITC filter set CCD camera (C11440 Orca Flash 4.0, Hamamatsu). Image sequences were analyzed using Fiji NIH image software.

Tonello R., Anderson W.B., Davidson S., Escriou V., Yang L., Schmidt B.L., Imlach W.L, & Bunnett N.W. (2022). The contribution of endocytosis to sensitization of nociceptors and synaptic transmission in nociceptive circuits. Pain, 164(6), 1355-1374.

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