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Sta neoplastine ci plus

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STA Neoplastine CI Plus is an automated coagulation reagent used for the determination of the prothrombin time (PT) in human citrated plasma. It is designed for use with the STA family of coagulation analyzers.

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Lab products found in correlation

Fibri prest automate, by Diagnostica Stago (6 mentions) Ptt automate, by Diagnostica Stago (6 mentions) Biochemistry panel plus analyzer discs, by Abaxis (5 mentions) Laser based hematologic analyzer, by Beckman Coulter (4 mentions) Piccolo point of care analyzer, by Abaxis (4 mentions) Sta thrombin, by Diagnostica Stago (4 mentions) Sta 4 clotting analyzer, by Diagnostica Stago (2 mentions) Sta ptt automate, by Diagnostica Stago (2 mentions) Sta fibrinogen, by Diagnostica Stago (1 mentions) Sta compact analyzer, by Diagnostica Stago (1 mentions) Lh750, by Beckman Coulter (1 mentions) Xe 2100, by Sysmex (1 mentions) Hemavet 950fs, by Drew Scientific (1 mentions) Start 4 hemostasis analyzer, by Diagnostica Stago (1 mentions) Point of care analyzer, by Abaxis (1 mentions) Sta compact, by Diagnostica Stago (1 mentions)

12 protocols using sta neoplastine ci plus

1

Comprehensive Hematological and Biochemical Analyses

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Total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin concentrations, mean cell volumes, mean corpuscular volumes, and mean corpuscular hemoglobin concentrations were analyzed from blood collected in tubes containing EDTA using a laser based hematologic analyzer (Beckman Coulter). Serum samples were tested for concentrations of albumin, amylase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), glucose, cholesterol, total protein, total bilirubin (TBIL), blood urea nitrogen (BUN), creatinine (CRE), and C-reactive protein (CRP) by using a Piccolo point-of-care analyzer and Biochemistry Panel Plus analyzer discs (Abaxis). Citrated plasma samples were analyzed for coagulation parameters prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen on the STart4 instrument using the PTT Automate, STA Neoplastine CI plus, and Fibri-Prest Automate, kits, respectively (Diagnostica Stago).
Thi E.P., Mire C.E., Lee A.C., Geisbert J.B., Zhou J.Z., Agans K.N., Snead N.M., Deer D.J., Barnard T.R., Fenton K.A., MacLachlan I, & Geisbert T.W. (2015). Lipid nanoparticle siRNA treatment of Ebola virus Makona infected nonhuman primates. Nature, 521(7552), 362-365.
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2

Comprehensive Hematological and Biochemical Analyses

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Total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin concentrations, mean cell volumes, mean corpuscular volumes, and mean corpuscular hemoglobin concentrations were analyzed from blood collected in tubes containing EDTA using a laser based hematologic analyzer (Beckman Coulter). Serum samples were tested for concentrations of albumin, amylase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), glucose, cholesterol, total protein, total bilirubin (TBIL), blood urea nitrogen (BUN), creatinine (CRE), and C-reactive protein (CRP) by using a Piccolo point-of-care analyzer and Biochemistry Panel Plus analyzer discs (Abaxis). Citrated plasma samples were analyzed for coagulation parameters prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen on the STart4 instrument using the PTT Automate, STA Neoplastine CI plus, and Fibri-Prest Automate, kits, respectively (Diagnostica Stago).
Thi E.P., Mire C.E., Lee A.C., Geisbert J.B., Zhou J.Z., Agans K.N., Snead N.M., Deer D.J., Barnard T.R., Fenton K.A., MacLachlan I, & Geisbert T.W. (2015). Lipid nanoparticle siRNA treatment of Ebola virus Makona infected nonhuman primates. Nature, 521(7552), 362-365.
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3

Comprehensive Blood Analysis Protocol

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Total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin concentrations, mean cell volumes, mean corpuscular volumes, and mean corpuscular hemoglobin concentrations were analyzed from blood collected in tubes containing EDTA using a laser-based hematologic analyzer (Beckman Coulter). Serum samples were tested for concentrations of albumin, amylase, ALT, AST, ALP, GGT, glucose, cholesterol, total protein, total bilirubin, BUN, CRE, and CRP by using a Piccolo point-of-care analyzer and Biochemistry Panel Plus analyzer discs (Abaxis). Citrated plasma samples were analyzed for coagulation parameters PT, APTT, thrombin time, and fibrinogen on the STart4 instrument using the PTT Automate, STA Neoplastine CI Plus, STA Thrombin, and Fibri-Prest Automate kits, respectively (Diagnostica Stago). Citrated plasma levels of D-dimers were measured by enzyme-linked immunosorbent assay according to the manufacturer's recommendations (Diagnostica Stago).
Thi E.P., Mire C.E., Ursic-Bedoya R., Geisbert J.B., Lee A.C., Agans K.N., Robbins M., Deer D.J., Fenton K.A., MacLachlan I, & Geisbert T.W. (2014). Marburg virus infection in nonhuman primates: Therapeutic treatment by lipid-encapsulated siRNA. Science translational medicine, 6(250), 250ra116.
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4

Comprehensive Blood Analysis Protocol

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Total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin concentrations, mean cell volumes, mean corpuscular volumes, and mean corpuscular hemoglobin concentrations were analyzed in blood specimens collected in tubes containing ethylenediaminetetraacetic acid, using a laser based hematologic analyzer (Beckman Coulter). Serum samples were tested for concentrations of albumin, amylase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ glutamyl transferase, glucose, cholesterol, total protein, total bilirubin, blood urea nitrogen, creatinine, and C-reactive protein by using a Piccolo point-of-care analyzer and Biochemistry Panel Plus analyzer discs (Abaxis). Citrated plasma samples were analyzed for coagulation parameters PT, APTT, thrombin time, and fibrinogen on the STart4 instrument using the PTT Automate, STA Neoplastine CI Plus, STA Thrombin, and Fibri-Prest Automate kits, respectively (Diagnostica Stago).
Cross R.W., Prasad A.N., Woolsey C.B., Agans K.N., Borisevich V., Dobias N.S., Comer J.E., Deer D.J., Geisbert J.B., Rasmussen A.L., Lipkin W.I., Fenton K.A, & Geisbert T.W. (2023). Natural history of nonhuman primates after conjunctival exposure to Ebola virus. Scientific Reports, 13, 4175.
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5

Modified Prothrombin Time Assay

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The prothrombin time (PT) assay was modified by dilution of test reagents essentially as done by de Maat and co-workers (34 (link)). PT reagent STA Neoplastine CI Plus (Diagnostica Stago) was diluted 1:500 with 16.6 mM CaCl2, and 0.1 ml of the diluted reagent was combined with 0.05 ml of normal human pooled plasma (NHPP) supplemented with purified AAT variants. Both test solutions were pre-warmed to 37°C prior to combination in a STA-IV clotting analyzer (Diagnostica Stago) and determination of clotting time.
Hamada M., Bhakta V., Andres S.N, & Sheffield W.P. (2021). Stepwise Reversion of Multiply Mutated Recombinant Antitrypsin Reveals a Selective Inhibitor of Coagulation Factor XIa as Active as the M358R Variant. Frontiers in Cardiovascular Medicine, 8, 647405.
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6

Fasting Blood Sampling for Metabolic Profile

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Blood samples were taken between 8.00 and 11.00 a.m. in a fasting state in order to monitor metabolic parameters. For blood counts, samples were obtained in EDTA-containing sterile tubes (S-Monovette® 1.2 mL, EDTA). Platelet counts were measured using Sysmex XE 2100, which has the capability to provide both an impedance platelet count and a fluorescence-based optical platelet count when the sample is run in the reticulocyte mode and Coulter LH-750, which uses impedance technology with enhanced data extraction techniques and algorithms to eliminate interference. For coagulation testing, samples were collected in citrate containing sterile tubes (S-Monovette® 1.4 mL, citrate). The activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen activity were measured on the STA-Compact analyzer (Diagnostica Stago, Paris, France) via mechanical clot detection using the following reagents: Sta-PTT Automate, Sta-Neoplastine CI plus, and Sta-Fibrinogen, all by Diagnostica Stago. Platelet counts are given in G/ L; APTT values in seconds; PT values are expressed as percentage of normal, fibrinogen activity given in mg/dL.
Dressler A., Chiara H., Benninger F., Waldhoer T., Gröppel G., Trimmel-Schwahofer P., Samueli S., Reithofer E., Elisa E., Feucht M, & Male C. (2020). Effects of the ketogenic diet on platelet counts and global coagulation tests in childhood epilepsy. Seizure, 80.
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7

Comprehensive Hematological and Coagulation Profile

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The total white blood cell count, lymphocyte, platelet and neutrophil cell counts were determined from EDTA blood with the HemaVet 950FS analyzer (Drew Scientific, Dallas, TX). Blood samples for coagulation parameter assays were collected into 1.8 mL citrate vacutainers. Plasma was separated by centrifugation and analyzed for activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), and fibrinogen concentration on a Start4 instrument using the PTT Automate, STA Neoplastine CI plus, STA Thrombin and Fibri-Prest Automate kits, respectively (Diagnostica Stago, Parsippany, NJ).
Marzi A., Banadyga L., Haddock E., Thomas T., Shen K., Horne E.J., Scott D.P., Feldmann H, & Ebihara H. (2016). A hamster model for Marburg virus infection accurately recapitulates Marburg hemorrhagic fever. Scientific Reports, 6, 39214.
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8

Coagulation Biomarkers in Clinical Trial

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Plasma fibrinogen concentration [Clauss method (Fibrinogen STAGO, Diagnostica Stago, Asnieres-sur-Seine, France)], aPTT (STA cephascreen STAGO, Diagnostica Stago, Asnieres-sur-Seine, France), PT and INR (STA Neoplastine CI Plus, Diagnostica Stago, Asnieres-sur-Seine, France) were performed with blood samples of approximately 3 ml collected by venipuncture into a tube with citrate (3.2%; Sarsted®, Wedel, Germany). The controls used were STA COAG control N+P, device: STA R evolution and STA Compact for coagulometric mothodology. Platelet count was performed on plasma samples with ethylenediamine tetraacetic acid (EDTA-tube; 3.2%; Sarsted®, Wedel, Germany) with E Check XE control (XE 2100, Sysmex, São Paulo, Brazil).
Coagulopathy was defined according to changes in CCT as follows: thrombocytopenia <150 x103/mm3, serum fibrinogen concentration <150 mg/dL or prolonged global coagulation time, such as INR >1.5 and TTPa >32 seconds [18 (link)].
Crochemore T., Corrêa T.D., Lance M.D., Solomon C., Neto A.S., Guerra J.C., Lellis P.S., Bernz L.M., Nunes N., Mancio C.M., Yokoyama A.P, & Silva E. (2018). Thromboelastometry profile in critically ill patients: A single-center, retrospective, observational study. PLoS ONE, 13(2), e0192965.
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9

Diluted PT Assay for AAT Variants

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The PT assay was modified by dilution of test reagents, similarly to the approach of de Maat et al.37 (link). PT reagent STA Neoplastine CI Plus (Diagnostica Stago) was diluted 1:500 with 16.6 mM CaCl2. Normal human pooled plasma (NHPP, 50 µL) was as previously described and was supplemented with purified AAT variants and pre-warmed to 37 °C prior to starting the reaction by addition of 100 µL of diluted PT reagent. A STA-IV clotting analyzer was employed (Diagnostica Stago) for all clotting assays in this study.
Bhakta V., Hamada M., Nouanesengsy A., Lapierre J., Perruzza D.L, & Sheffield W.P. (2021). Identification of an alpha-1 antitrypsin variant with enhanced specificity for factor XIa by phage display, bacterial expression, and combinatorial mutagenesis. Scientific Reports, 11, 5565.
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10

Evaluating EMV Coagulation Potency

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PT and CT were applied to assess the potency of EMVs to support coagulation. To perform PT (11), 25 μL normal pooled plasma (NPP) was mixed with 25 μL EMVs and incubated for 60 sec at 37 °C. 100 μL PT solution (Diagnostica Stago Inc STA-Neoplastine CI Plus) was next added to the mixture and the time of coagulation was recorded. We used a semi-automated benchtop system (Diagnostica Stago STart® 4 Hemostasis Analyzer, USA) to accomplish both the assays. For CT, we followed the procedure of a previously published study.12 (link) Additionally, at the end of the storage time (day 42), we carried out an extra PT assay using serial dilutions of EMVs samples in PBS including 1:2, 1:4, 1:18, 1:16, and 1:32. The first tube contained 50 μL NPP and 50 μL PBS. The other tubes contained 50 μL NPP and 50 μL of each EMVs dilution, respectively. After adding 200 μL PT solution to the mixtures, the coagulation time of each tube was defined.
Roshanzamir F., Amini-Kafiabad S., Zarif M.N., Arabkhazaeli A, & Mohammadipour M. (2022). The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward. European Journal of Translational Myology, 32(3), 10708.
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