Antibody against cd68

AG1478 were purchased from Sigma-Aldrich (St. Louis, MO). Compound 542 (Fig. 1a) was prepared with a purity of 99.2% as described in our previous study34 (link). AG1478 and compound 542 were dissolved in dimethyl sulfoxide (DMSO) for in vitro experiments and in 1% sodium carboxyl methyl cellulose (CMC-Na) for in vivo experiments. Antibodies against GAPDH, p-EGFR and p-AKT were purchased from Cell Signaling (Danvers, MA, USA). Antibodies against p-ERK, TGF-β, Collagen4, cleaved caspase 3, Bax, Bcl-2, and TLR4 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against CD68 was purchased from Abcam (Cambridge, MA). Human vascular smooth muscle cell line was purchased from R&S Biotech. Co., LTD (Shanghai, China).

Human recombinant TNFα protein was purchased from PeproTech (Rocky Hill, NJ, USA). Src-inhibitor saracatinib, JAK-inhibitor tofacitinib, mitogen-activated protein kinase-inhibitor SB203580, STAT3-inhibitor S3I-201, p65-inhibitor JSH-23, IKK-inhibitors TPCA-1 and IKK-16 were perchased from Selleckchem (Houston, TX, USA), JAK-inhibitor PDTC was perchased from Abcam (Cambridge, MA, USA). Antibody against HK2, phospho-IKKα/β (Ser176/180) and phospho-IKKε (Ser172) were purchased from Cell Signaling Techonology (Beverly, MA, USA), antibody against YAP was purchased from NOVUS Biologicals (Littleton, CO, USA), antibody against CD68 was pursed from Abcam, antibody against p65 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against β-actin, GFP, Myc, Flag, HA and TEAD4 were purchased from Sigma-Aldrich. Antibody against TNFR1, IKKα, IKKβ and IKKε were purchased from Abclonal Technology (Wuhan, China). Antibody against pan-phosphorylated serine/threonine was purchased from BD Biosciences (San Jose, CA, USA).

Chlorpyrifos (CAS no. 2921-88-2) 98% purity was purchased from Chem Service and diluted in 10% ethanol and 90% peanut oil (Sigma). Paraformaldehyde (PFA) was purchased from Electron Microscopy Sciences and diluted to working concentrations in PBS. An antibody against APP (APP C-terminal) obtained from ThermoFisher Scientific was used at dilutions of 1:1000 for western blot (WB). An antibody against abnormally phosphorylated tau (clone tau-ps199/202) was obtained from Sigma and used at a concentration of 1:1000 for immunohistochemistry (IHC) and 1:4000 for WB. An antibody against Iba1 (1:5000 for IHC) was obtained from Wako Ltd., and an antibody against CD68 (1: 1000 for IHC) was obtained from Abcam. β-Actin (1:1000 for WB) was obtained from Santa Cruz. Secondary antibodies against mouse and rabbit (1:5000–1:10,000 for WB) were obtained from Cell Signaling, and secondary ImmPRESS IgG HRP and ImmPACT Nova Red and DAB kits were obtained from Vector. Congo Red was obtained from Sigma.