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Uvp uvgl 25

Manufactured by Analytik Jena

The UVP UVGL-25 is a compact, multi-wavelength UV lamp designed for general laboratory use. It provides near-ultraviolet (UV) and visible light illumination for various applications, including viewing fluorescent materials, performing thin-layer chromatography (TLC), and other analytical procedures requiring UV or visible light sources.

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2 protocols using uvp uvgl 25

1

Characterization of CB-CD Fluorescent Nanoparticles

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The channel precision
oven was used
for hydrothermal heating treatment. The FL absorption color of CB-CDs
was checked by using UV light irradiation with a long excitation wavelength
at 365 nm (Analytik Jena UVP UVGL-25). The FL spectra were recorded
by using FL spectroscopy (Varian Cary Eclipse Fluorescence Spectrophotometer).
FT-IR spectrum was obtained by using FT-IR spectroscopy (ALPHA FT-IR
Spectrometer from Bruker). The absorption spectra were recorded by
UV–vis spectroscopy (Spectra Academy UV–Vis Spectrometer
Detector SV-2100). The TEM images were scanned by a Hitachi HT-7700
microscope and JEM-3010 HR-TEM (JEOL, Japan). The Raman spectrum was
recorded on a Micro Raman Identify Spectrometer (ProTrusTech Co. Ltd.).
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2

Synthesis of HEAA-based Microgels

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Microgels were prepared by mixing 70 mL hexane, 500 μL SpanTM80 and 100 μL TWEEN®80 in a rubber plug-sealed flask and stirred vigorously under a nitrogen-rich atmosphere for 30 min. Polymer precursor solutions were prepared by mixing 1 M of HEAA with 0–10 mol% of DMA, 6 mol% of MBAA and 4 mol% of VA086 relative to HEAA in DI water. The precursor solutions were frozen for 30 min and degassed 3 times by backfilling with N2. Then the precursor solutions were added to the surfactant mixture drop by drop using a syringe. The reaction was initiated by irradiating UV light (365 nm, UVP UVGL-25, Analytik Jena) on the side of the flask and rigorous stirring for 4 h (Scheme S1). The microgels were collected by filtration and washed with acetone and isopropyl alcohol in succession until the microgels became white. The microgels were dried under vacuum for overnight and further washed with DI water (pH 3.5) for 30 min. Microgels were collected through centrifugation at 5,000 rpm for 10 min and lyophilized to yield dried microgel powder.
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