Monoclonal antibodies against tubulin

Monoclonal antibodies against Snail-1, ERK1/2, phospho-ERK1/2, phospho-SMAD1-5-8, and Cav1 were from Cell Signaling Technology; monoclonal antibodies against E-cadherin, β-catenin, and γ-catenin were from BD (Becton-Dickinson Laboratories, Mountain View, CA); monoclonal antibodies against occludin was from Invitrogen (Carlsbad, CA); monoclonal antibodies against tubulin, α-SMA, α-catenin, vimentin, pan-cytokeratin, and fibronectin were from Sigma (Saint Louis, MO); monoclonal anti-N-cadherin and polyclonal anti-ZO-1 were from Zymed (Invitrogen, Carlsbad, CA); polyclonal anti-actin, -SMAD2-3, and -phospho-MEK were from Santa Cruz Biotechnology (CA); and polyclonal anti-phospho SMAD2-3 was from Biosource (Camarillo, CA). Monoclonal anti-CD54 was from Biolegend (San Diego, CA); polyclonal anti-Fsp1 was from Dako (Glostrup, Denmark); monoclonal anti-CD31 from Serotec (Oxford, UK); and polyclonal anti-Ki67 from Abcam (Cambridge, UK). Monoclonal anti-JAM-A clone BV-11 was a gift from Dr. E. Dejana (Milan Italy). Fluor 647?phalloidin and Hoechst 33342 were from Invitrogen (Carlsbad, CA). CI 1040 was from Selleck (Houston, TX).

Polyclonal antibodies against phospho-STAT3 (Tyr705) phospho-NFκB (Ser536) were from Cell Signaling (Cell Signaling Technology, Danvers, MA); monoclonal antibodies against tubulin were from Sigma (Saint Louis, MO). Recombinant human IL-6 ad soluble IL-6 receptor (sIL-6R) were from R&D Systems (Minneapolis, MN). LPS was from Sigma Aldrich, (St Louis, MO) CpG-DNA, Pam3CSK4, Muramyl dipeptide (MDP) and Poly(IC) were from Invivogen (San Diego, CA); S100A8 and S100A9 were from Vogl lab. TCZ (RoActemra) was from Roche, Milan, Italy.