Cell pellets were lysed using RIPA buffer (Fisher Scientific) supplemented with protease and phosphatase inhibitors (Sigma). 20-30μg protein per sample was run on a polyacrylamide gel and transferred to a PVDF membrane. Proteins were detected using respective primary and HRP-conjugated secondary antibodies, including rabbit anti-IGF1R (Cell Signaling 3027 1:500), rabbit anti-ZWILCH (Abcam 202898 1:1,000), mouse anti-NCL (Abcam 136649 1:1,000), rabbit anti-AUF1 (Cell Signaling 12382 1:1,000), rabbit anti-ELAVL1 (Cell Signaling 12582), rabbit anti-KHSRP (Cell Signaling 5398 1:500), rabbit anti-TIAL1 (Cell Signaling 5137 1:1,000), mouse anti-β-actin (Sigma A5316 1:1,000), goat anti-rabbit (Invitrogen 31460 1:5,000), and goat anti-mouse (Invitrogen 31430 1:5,000).
Mouse anti ncl
Manufactured by Abcam
Mouse anti-NCL is a primary antibody that recognizes the nucleolin (NCL) protein. Nucleolin is a multifunctional protein involved in various cellular processes such as ribosome biogenesis, chromatin remodeling, and regulation of gene expression. This antibody can be used to detect and study the NCL protein in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Protease and phosphatase inhibitor, by Merck Group (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Rabbit anti zwilch, by Abcam (2 mentions)
Rabbit anti auf1, by Cell Signaling Technology (2 mentions)
Rabbit anti elavl1, by Cell Signaling Technology (2 mentions)
Rabbit anti khsrp, by Cell Signaling Technology (2 mentions)
Rabbit anti tial1, by Cell Signaling Technology (2 mentions)
Rabbit anti igf1r, by Cell Signaling Technology (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
2 protocols using mouse anti ncl
1
Western Blot Protein Detection Protocol
2
Western Blot Protein Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell pellets were lysed using RIPA buffer (Fisher Scientific) supplemented with protease and phosphatase inhibitors (Sigma). 20-30μg protein per sample was run on a polyacrylamide gel and transferred to a PVDF membrane. Proteins were detected using respective primary and HRP-conjugated secondary antibodies, including rabbit anti-IGF1R (Cell Signaling 3027 1:500), rabbit anti-ZWILCH (Abcam 202898 1:1,000), mouse anti-NCL (Abcam 136649 1:1,000), rabbit anti-AUF1 (Cell Signaling 12382 1:1,000), rabbit anti-ELAVL1 (Cell Signaling 12582), rabbit anti-KHSRP (Cell Signaling 5398 1:500), rabbit anti-TIAL1 (Cell Signaling 5137 1:1,000), mouse anti-β-actin (Sigma A5316 1:1,000), goat anti-rabbit (Invitrogen 31460 1:5,000), and goat anti-mouse (Invitrogen 31430 1:5,000).
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