Alexa fluor 594 conjugated goat anti rabbit or anti mouse igg antibodies

Manufactured by Jackson ImmunoResearch

Alexa Fluor® 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies are fluorescently labeled secondary antibodies. They are designed to detect and visualize primary antibodies raised in rabbit or mouse, respectively, in various immunological applications.

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3 protocols using alexa fluor 594 conjugated goat anti rabbit or anti mouse igg antibodies

Comprehensive Immunofluorescence and Western Blot Analysis

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The following antibodies were used: anti-Ki67 (ab16667, Abcam; IF), anti-BrdU (347580, Biosciences; IF), anti-filaggrin (PRB-417, Covance; IF), anti-involucrin (RINVOL, 924401, Biologend; IF), anti-K5 (SAB45016501, Sigma-Aldrich; IF), anti-K10 (sc-23877, Santa Cruz Biotechnology; IF), anti-K16 (sc-53255, Santa Cruz Biotechnology; IF), anti-Cyclin A (sc-751, Santa Cruz Biotechnology; IF), anti-pH3 (sc-8656-R, Santa Cruz Biotechnology; IF), anti-p-H2AX (sc-517348, Santa Cruz Biotechnology; IF), anti-p40 (deltaNp63, 24-8626-RBP1, ARP; IF), anti-Cyclin E1 (sc-248, Santa Cruz Biotechnology; WB), anti-PCNA (sc-56, Santa Cruz Biotechnology; WB) and anti-GAPDH (sc-32233, Santa Cruz Biotechnology; WB).The following secondary antibodies were used: Alexa Fluor® 488-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Jackson; IF), Alexa Fluor® 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Jackson; IF) and DyLight^TM 800-conjugated goat anti-mouse IgG antibody (Invitrogen; WB).

Contreras L., García-Gaipo L., Casar B, & Gandarillas A. (2024). DNA damage signalling histone H2AX is required for tumour growth. Cell Death Discovery, 10, 99.

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Antibody Characterization for Epidermal Differentiation

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The following antibodies were used: anti-Cdk1 (610038, BD Biosciences; immunohistochemistry (IHC)), anti-filaggrin (PRB-417P, Covance; immunofluorescence (IF)), anti-involucrin (RINVOL, 924401 Biolegend; FC and IF; SY3 [65 (link)], FC), anti-IgG (I-5381, Sigma-Aldrich; FC), anti-K1 (PRB-149P, Covance; FC), anti-K5 (SAB45016501, Sigma-Aldrich; IF), anti-K10 (C7284, Sigma-Aldrich; FC, sc-23877, Santa Cruz Biotechnology; IF and PRB-159P, Covance; IF), anti-K13 (sc-390982, Santa Cruz Biotechnology; IF), anti-K16 (LL025, Santa Cruz Biotechnolog; FC), anti-Ki67 (sc-7844, Santa Cruz Biotechnology; IF), anti-Plk1 (laboratory made; [18 (link), 23 (link)] IHC), anti-phospho-histone H3 Ser10 (9701, Cell Signalling; IHC) and anti-PCNA (sc-56, Santa Cruz Biotechnology; IF).
The following secondary antibodies were used: Alexa Fluor® 488-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Jackson; FC and IF), Alexa Fluor® 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Jackson; IF).

Sanz-Gómez N., de Pedro I., Ortigosa B., Santamaría D., Malumbres M., de Cárcer G, & Gandarillas A. (2020). Squamous differentiation requires G2/mitosis slippage to avoid apoptosis. Cell Death and Differentiation, 27(8), 2451-2467.

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Antibody Profiling of Epidermal Markers

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The following primary antibodies from Santa Cruz Biotechnology were used: anti-FOXM1 (Western Blot: WB), anti-GAPDH (FL-335, WB), anti-keratin K10 (RKSE60, immunofluorescence: IF), anti-p53 (FL-393, IF and WB), anti-keratin K16 (Flow Cytometry: FC and WB) and anti-wee1 (B11, WB). Other antibodies used were: anti-involucrin (SY5, Sigma-Aldrich, IF, FC; SY3^{54 (link)}, WB), anti-BrdU (BD Biosciences, FC), anti-filaggrin (PRB-417P, COVANCE, WB), anti-γH2AX (Ser139, Millipore, FC and WB), anti-keratin K1 (Poly19052, Biolegend, IF, FC), keratin K13 (KS-1A3, NOVUS, FC) and anti-p21 (P1484, Sigma-Aldrich, WB). Keratins K16 and K13 are typical of hyperplastic skin and oral epithelium, respectively and both are expressed in suprabasal cultured keratinocytes^{12 (link),21}.
The following secondary antibodies from Jackson ImmunoResearch were used: Alexa Fluor® 488-conjugated goat anti-rabbit or anti-mouse IgG antibodies (FC and IF); Alexa Fluor® 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies (IF). Other secondary antibodies used were: IRdye800-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Li-Cor, WB) and HRP-conjugated goat anti-rabbit or anti-mouse IgG antibodies (Bio-Rad, WB).

de Pedro I., Alonso-Lecue P., Sanz-Gómez N., Freije A, & Gandarillas A. (2018). Sublethal UV irradiation induces squamous differentiation via a p53-independent, DNA damage-mitosis checkpoint. Cell Death & Disease, 9(11), 1094.

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