Nis elements software version br 3

To evaluate the effect of gene transfer on periodontal repair, digital images of H&E-stained specimens were captured using a Nikon Eclipse E800 microscope (Nikon, Inc., Melville, NY, USA) fitted with a SPOT-2 camera (Diagnostic Instruments, Inc, Sterling Heights, MI, USA) for analysis using NIS-Elements software version BR-3.2 (Nikon Instruments, Inc., Melville, NY, USA). Images were captured at 20, 40, 100, 200, and 400x magnification. A single masked, calibrated examiner (ABP) completed the histomorphometric analysis. The examiner’s pre- and post-study calibration inter- and intra-examiner error was <5% compared to a standard (WVG). The parameters measured included ^{37 (link)}: (1) defect length, (2) new bridging bone length (mm), measured from the borders of the surgically-created osseous defect (mesiodistally); (3) bridging bone percentage of defect length, (4) new alveolar bone formed within the defect, (5) alveolar bone defect fill, determined as the percentage of new bone in the original bone defect area excluding the PDL space, (6) length of prepared root surface, and (7) length of new cementum formed along the prepared root surface.