To measure reactive oxygen species (ROS) production, 10 μM 2,7-dichlorofluorescein diacetate (DCFDA; Sigma-Aldrich, Burlington, MA, USA, D6883) was added to cell cultures for 15 min. Then, cells were detached by Trypsin-EDTA solution (Aurogene, Rome, Italy), washed with PBS 1X and analyzed by FACScalibur flow cytometer (BD Transduction Laboratories, Franklin Lakes, NJ, USA) using CELLQuest Pro-software (version 6.0, BD Biosciences, Franklin Lakes, NJ, USA). For each analysis, 10,000 events were recorded.
Trypsin edta solution
Manufactured by Aurogene
Sourced in Italy
Trypsin-EDTA solution is a widely used reagent in cell culture applications. It is a mixture of the enzyme trypsin and the chelating agent EDTA. The primary function of this solution is to facilitate the dissociation and detachment of adherent cells from the culture surface, enabling the passaging and sub-culturing of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (1 mentions)
Cellquest pro software, by BD (1 mentions)
Fetal bovine serum (fbs), by Aurogene (1 mentions)
Resazurin sodium salt, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Aurogene (1 mentions)
Dulbecco s modified eagle medium dmem, by Lonza (1 mentions)
Sodium chloride (nacl), by Duchefa Biochemie (1 mentions)
Tryptone, by Duchefa Biochemie (1 mentions)
Yeast extract, by Duchefa Biochemie (1 mentions)
2 protocols using trypsin edta solution
1
Measuring Cellular Reactive Oxygen Levels
2
Alginate-Based Curcumin Formulation Development
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Sodium alginate (Ph.Eur. grade, MW 180-300 kDa, Lot. 9C260/DOC) was purchased from Carlo Erba reagents (Milano, Italy). Yeast extract, tryptone, NaCl and antibiotics for E. coli growth were from Duchefa Biochemie. For cell culture, Dulbecco's modified eagle medium (DMEM) was purchased from Lonza Group (Basel, Switzerland), fetal bovine serum (FBS), penicillin/streptomycin solution and trypsin/ EDTA solution were from Aurogene (Rome, Italy), and Resazurin sodium salt was from Alfa Aesar (Ward Hill, MA, USA). Curcumin, 2, 2diphenil-1-picrylhydrazyl (DPPH) and all the other products and chemicals used during the experiments were obtained from Sigma Aldrich (St. Louis, USA) and were of reagent grade with the highest purity available. Deionized ultra-filtered water was used throughout this study.
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