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3 protocols using dipotassium phosphate

1

Casein and Gelatin Protocol

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Casein was purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Gelatin, pure amino acids (L form), Minimal Davis Broth (Ingredients g/L: Dipotassium phosphate, 7.0 g; Monopotassium phosphate, 2.0 g; Sodium citrate, 0.5 g; Magnesium sulphate, 0.1 g; Ammonium sulphate,1.0 g) and other media components were purchased from Hi-Media Laboratories (Mumbai, India). All other chemicals used were of highest purity grade.
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2

Phytochemical Analysis and Antioxidant Evaluation

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Murashige and Skoog (MS) medium, kinetin, naphthalene acetic acid, pectin, MnCl2, methanol, toluene, chloroform, ethanol, diethyl ether, benzene, streptomycin, ciprofloxacin, doxycycline, ampicillin, ofloxacin, dimethyl sulfoxide, DPPH (1,1-diphenyl-2-picrylhydrazyl), sodium acetate, glacial acetic acid, hydrochloric acid, TPTZ (2,4,6-tri[2-pyridyl]-s-triazine), ferrous sulphate, ferric chloride, nitro blue tetrazolium, ethylenediaminetetraacetic acid (EDTA), riboflavin, hydrogen peroxide, methionine, dipotassium phosphate, monopotassium phosphate, and thymol (THY) were purchased from HIMEDIA; thymoquinone (TQ) was purchased from Sigma Aldrich.
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3

Xylan and Arabinoxylan Extraction Protocol

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Kanamycin, ampicillin, and IPTG were purchased
from Sigma-Aldrich
Co. LLC., USA. The analytical-grade reagents and chemicals, namely,
sodium chloride, sodium acetate, glucose, yeast, extract, peptone,
tryptone, di-potassium phosphate, monopotassium phosphate, glycerol,
sodium carbonate, sodium bicarbonate, sodium potassium tartrate, sodium
sulfate, copper sulfate, ammonium molybdate, trifluoroacetic acid
(TFA), Coomassie Brilliant Blue G-250, and sodium arsenate were purchased
from Himedia Pvt. Ltd., India. The xylan substrate was purchased from
Carbosynth, UK, and rye arabinoxylan from Megazyme, Ireland.
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