Human renal cortex proximal convoluted tubule epithelial cells HK‐2 (Procell Life Science & Technology Co., Ltd.); DMEM‐12 medium (KGM12500S; KeyGen bioTECH); Lipofectamine Reagent 3000 Transfection (L3000015; Invitrogen); Cell Counting Kit‐8 (KGA317; KAIJI Biotech); Reporter plasmid pmirGLO, Plasmid Extraction Kit (DP103‐02; TIANGEN Biotech); Annexin V‐FITC/PI Apoptosis Kit (AP101‐100‐kit; MULTI SCIENCES); internal reference antibody: rabbit anti‐β‐actin (AF7018, Affinity); target primary antibodies: anti‐p‐PI3K (rabbit, AF3241, Affinity), anti‐p‐AKT (rabbit, AF0832, Affinity), anti‐Bax (rabbit, AF0120, Affinity), anti‐Bcl‐2 (rabbit, AF6139, Affinity), and anti‐Cleaved Caspase‐3 (rabbit, AF7022, Affinity).
Annexin 5 fitc pi apoptosis kit ap101 100 kit
Manufactured by MultiSciences Biotech
Sourced in China
The Annexin V‐FITC/PI Apoptosis Kit (AP101‐100‐kit) is a laboratory tool used for the detection and quantification of apoptosis in cell samples. It contains reagents for the fluorescent labeling of cells, allowing for the identification of cells undergoing apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Af7018, by Affinity Biosciences (1 mentions)
Af0832, by Affinity Biosciences (1 mentions)
Af0120, by Affinity Biosciences (1 mentions)
Af6139, by Affinity Biosciences (1 mentions)
Af7022, by Affinity Biosciences (1 mentions)
Af3241, by Affinity Biosciences (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Cell cycle staining kit ccs012, by MultiSciences Biotech (1 mentions)
Ultrasybr mixture cw0957m, by CWBIO (1 mentions)
Goat anti rabbit igg zb 2301, by ZSGB-BIO (1 mentions)
Goat anti mouse igg zb 2305, by ZSGB-BIO (1 mentions)
Hifiscript first strand cdna synthesis kit cw2569m, by CWBIO (1 mentions)
Bca protein quantification kit, by CWBIO (1 mentions)
Fluorescence activated cell sorting (facs), by BD (1 mentions)
3 protocols using annexin 5 fitc pi apoptosis kit ap101 100 kit
1
Apoptosis Regulation in Renal Tubular Cells
2
Cell Cycle and Apoptosis Analysis
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Cell cycle staining kit (CCS012) and Annexin V-FITC/PI apoptosis kit (AP101-100-kit) were purchased from Multi-Sciences, Beijing, China. Trizon Reagent (CW0580S), ultrapure RNA extraction kit (CW0581M), HiFiScript first strand cDNA synthesis kit (CW2569M), miRNA purification and reverse transcription kit (CW0627S), ultraSYBR mixture (CW0957M) and BCA protein quantification kit (CW0014S) were products of CWBIO, Beijing, China. Ultrasensitive luminescence solution (RJ239676) was from Thermo Fisher, USA. Mouse monoclonal antibody against GAPDH (TA-08, 1:1,000), goat anti rabbit IgG (ZB-2301, 1:500) and goat anti mouse IgG (ZB-2305, 1:500) were purchased from Zsbio, Beijing, China. Mouse monoclonal antibody against P53 (cat no. 2524) was obtained from Cell Signaling, USA. Rabbit monoclonal antibody against Bax (ab32503) and polyclonal antibody against Bcl-2 were from Abcam, UK and Bioss, USA, respectively.
Flow cytometer (NovoCyteTM) was from Eisen Biologicals, Hangzhou, China. Fluorescent PCR instrument (CFX Connect™) and ultrasensitive chemiluminescence imaging system (Chemi DocTM XRS+) were purchased from Bio-Rad, Shanghai, China.
Flow cytometer (NovoCyteTM) was from Eisen Biologicals, Hangzhou, China. Fluorescent PCR instrument (CFX Connect™) and ultrasensitive chemiluminescence imaging system (Chemi DocTM XRS+) were purchased from Bio-Rad, Shanghai, China.
3
Annexin V-FITC/PI Apoptosis Assay
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After receiving HSP under the optimal condition and incubating for 72 h, BMSCs were washed with PBS three times and collected. Annexin V-FITC/PI double staining assay was performed by utilizing the Annexin V-FITC/PI Apoptosis Kit (AP101-100-kit, MULTI SCIENCES) according to the manufacturer’s instructions. Briefly, 195 μL binding buffer and 5 μL Annexin V-FITC were added and incubated at room temperature away from light for 10 min. Next, 200 μL binding buffer that contained 10 μL PI was added and incubated at room temperature away from light for 15 min. The samples were immediately analyzed using FACS (Becton, Dickinson and Company, Franklin Lake, NJ, USA). The equation (number of cells in the upper right quadrant + cells in the lower right quadrant) / (total cell number) was used to calculate the apoptotic rate.
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