Skmm2

XGs human myeloma cell lines (HMCLs) were obtained as previously described 8 (link). AMO-1, LP1, L363, OPM2, MOLP2, MOLP8, Lopra and SKMM2 were purchased from DSMZ (Braunsweig, Germany) and RPMI8226 from ATCC (American Tissue Culture Collection, Rockville, MD, USA). JJN3 was kindly provided by Dr. Van Riet (Bruxelles, Belgium) and MM1S by Dr. S. Rosen (Chicago, USA). HMCLs were authenticated according to their short tandem repeat profiling and their gene expression profiling using Affymetrix U133 plus 2.0 microarrays deposited in the ArrayExpress public database under accession numbers E-TABM-937 and E-TABM-1088. HMCLs characteristics, obtained from previously published analysis results 8 (link), are available in Table S1. EBV-immortalized B-cells from 8 different patients have been used as control cells. The patients are those from whom the XG1, XG3, XG5, XG10, XG13, XG14, XG16 and XG19 cell lines were generated.

All (HMCLs) were previously characterized.^12,22 (link) The HMCLs BCN; NAN1, −3, −7, −8 and −9; SBN; and XG1, −2, −5, −6, −7 and −11 were derived in Nantes or Montpellier laboratories in the presence of interleukin-6 (IL-6). KMS11, KMS12-PE and KMM1 HMCLs were kindly provided by Dr. Otsuki (Kurashiki, Japan), JJN3 was kindly provided by Dr. Van Riet (Brussels, Belgium), JIM3 was kindly provided by Dr. MacLennan (Birmingham, UK), Karpas 620 was kindly provided by Dr. Karpas (Cambridge, UK) and MM1S was kindly provided by Dr. Rosen (Chicago, IL). AMO1, LP1, L363, NCI-H929, SKMM2, U266 and OPM2 were purchased from DSMZ (Braunsweig, Germany). All HMCLs were cultured in RPMI1640 supplemented with 5% fetal calf serum (FCS). Cell cultures from BCN, NAN, SBN and XG cells were additionally supplemented with 3 ng/mL IL-6. Blood or bone marrow samples from patients were collected after informed consent at the Department of Hematology, University Hospital of Nantes. Experiments with primary myeloma cells were carried out using unpurified or purified (CD138⁺) myeloma cells, depending on the myeloma infiltration and the total number of cells. Chromosome abnormalities were assessed by fluorescence in situ hybridization (FISH).

XG1, XG2, XG3, XG4, XG5, XG6, XG7, XG10, XG11, XG12, XG13, XG14, XG16, XG19, XG20 and XG21 HMCLs were obtained as previously described.^{36 (link)} AMO-1, LP1, L363, U266, OPM2 and SKMM2 were purchased from DSMZ (Braunschweig, Germany) and RPMI8226 from ATTC (Rockville, MD, USA). HMCLs were authenticated according to their short tandem repeat profiling and their gene expression profiling using Affymetrix U133 plus 2.0 microarrays deposited in the ArrayExpress public database under accession numbers E-TABM-937 and E-TABM-1088.^{36 (link)}