The anesthesia, intubation, and establishment of vascular catheters were performed as described previously (17 (
link), 18 (
link)). Then, animals were easily equipped with intracerebral probes. Three 0.8-mm-diameter burr holes were drilled in three quadrants centered in the bregma of the skull with access to the superficial cerebral cortex (
Fig. 1b). A CMA70 microdialysis catheter (CMA Microdialysis AB, Solna, Sweden) was inserted 4-mm deep into hole E to collect the dialysate from the cerebral cortex (
Fig. 1b). An intraparenchymal microtip pressure catheter (Codman ICP Express, Raynham, MA) was placed 3-mm deep into the cerebral cortex through hole C after puncture of the dura (
Fig. 1b). ICP was recorded by connecting the catheter to the transducer of a multichannel physiologic recorder (
MP150, BIOPAC Systems, Inc., California, USA), and the recordings were analyzed using the
AcqKnowledge system (Version 3.8.1, BIOPAC Systems, Inc). Hole D (
Fig. 1b) was devoted to monitoring the cerebral temperature with a microprobe (Licox Integra, Plainsboro, NJ) connected to the transducer of a multichannel physiologic recorder (
MP150, BIOPAC Systems, Inc. California, USA).
Tang Z., Liang M., Chen G., Rong J., Yao J., Chen Z., Yang X, & Wu Z. (2018). Neuroprotective effect of selective antegrade cerebral perfusion during prolonged deep hypothermic circulatory arrest: Cerebral metabolism evidence in a pig model. Anatolian Journal of Cardiology, 19(1), 2-10.