Log In Sign up
The largest database of trusted experimental protocols

Il 1α

Manufactured by R&D Systems
Visit Supplier
Sourced in United States, United Kingdom, Sweden

IL-1α is a cytokine that plays a role in the inflammatory response. It functions as a mediator of the host response to infection, immune responses, and trauma.

Automatically generated - may contain errors

Lab products found in correlation

Tnf α, by R&D Systems (31 mentions) Il 1β, by R&D Systems (25 mentions) Interleukin 6 (il 6), by R&D Systems (25 mentions) Tgf β, by R&D Systems (8 mentions) Il 17a, by R&D Systems (7 mentions) Il 33, by R&D Systems (6 mentions) Il 10, by R&D Systems (6 mentions) Il 18, by R&D Systems (5 mentions) Il 22, by R&D Systems (5 mentions) Ifn γ, by R&D Systems (5 mentions) Cxcl1, by R&D Systems (5 mentions) Tnf α, by Thermo Fisher Scientific (5 mentions) Tgf β1, by R&D Systems (4 mentions) Gm csf, by R&D Systems (4 mentions) Interleukin 4 (il 4), by R&D Systems (4 mentions) Il 13, by R&D Systems (4 mentions) Bioplex multiplex cytokine assay system, by Bio-Rad (3 mentions) Il 17f, by BioLegend (3 mentions) Hmgb1, by R&D Systems (3 mentions) Il 15, by R&D Systems (3 mentions)

97 protocols using il 1α

1

Cytokine and CM Effects on hPSM and A549

Check if the same lab product or an alternative is used in the 5 most similar protocols
hPSMs at passages 2–5 were used in our studies. All experiments were performed with FBS-free media at 95% culture confluence with a stable ratio of supernatant volume-to-surface available for cell adhesion (1.5 mL:9.6 cm2).
hPSMs and A549 cells were either untreated or treated for 6 or 48 h with: (a) 830 μg/mL CM, (b) 83 μg/mL CM, (c) a combination of 5 ng/mL IL-1α (R&D Systems, Minneapolis, MN, USA) and 50 ng/mL TNF-α (R&D Systems, Minneapolis, MN, USA), (d) a combination of 5 ng/mL IL-1α and 50 ng/mL TNF-α and 830 μg/mL CM, (e) a combination of 5 ng/mL IL-1α and 50 ng/mL TNF-α and 83 μg/mL CM, (f) 5 ng/mL TGF-β (R&D Systems, Minneapolis, MN, USA), (g) 5 ng/mL TGF-β and 830 μg/mL CM or (h) 5 ng/mL TGF-β and 83 μg/mL CM. At the end of the 6 h incubation period, hPSMs and A549 cells were lysed with Nucleozol Nucleozol (MACHEREY-NAGEL, Düren, Germany) and kept at −80 °C until assayed. At the end of the 48 h incubation period, supernatants and total cells were collected and kept at −80 °C until assayed.
Filidou E., Kandilogiannakis L., Tarapatzi G., Spathakis M., Steiropoulos P., Mikroulis D., Arvanitidis K., Paspaliaris V, & Kolios G. (2022). Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells. International Journal of Molecular Sciences, 23(9), 4570.
+ Open protocol
+ Expand
2

Modulation of IL-1α-Induced Cartilage Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Many studies of cartilage explants treated with IL-1 have been reported utilizing IL-1 concentrations ranging from 0.05 to 100ng/ml15 (link),24 (link). Based on our own preliminary dose-response study of sGAG-loss versus IL-1α (R&D Systems, Minneapolis, MN)) at 1, 2, 5, and 10ng/ml) with bovine cartilage, we chose an IL-1α concentration of 1ng/ml for all the present experiments, representing a moderately aggressive cytokine treatment. We then tested the effects of Dex (Sigma) alone (at 10nM, 100nM and 1mM) and IGF-1 (R&D Systems) alone (at 10, 100 and 300ng/ml) on sGAG loss and proteoglycan biosynthesis in bovine cartilage treated with l ng/ml IL-lα. Based on the results of these dose response studies (Fig. 1A,B), we chose concentrations of 100nM Dex and 100ng/ml IGF-1, both alone and in combination, for all subsequent tests of sGAG loss, biosynthesis, gene expression and cell viability.
Li Y., Wang Y., Chubinskaya S., Schoeberl B., Florine E., Kopesky P, & Grodzinsky A.J. (2014). Effects of Insulin-like Growth Factor-1 and Dexamethasone on Cytokine-Challenged Cartilage: Relevance to Post Traumatic Osteoarthritis. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 23(2), 266-274.
+ Open protocol
+ Expand
3

Humoral Factors Modulate Bone Marrow Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess the contributions made by humoral factors, 5-wk-old CAG-eGFP mice were treated with TPO (Sigma-Aldrich) or IL-1α (R&D Systems or BioLegend) at dosages of 10 µg/mouse subcutaneously (SC) daily for 5 d or 70 µg/mouse SC daily for 3 d. The mice were visualized and examined at 6-wk-old, 7 d after the first treatment. Some mice were also treated with neutralizing antibodies against IL-1α (R&D Systems or BioLegend), IL-1R (BioLegend), isotype-matched control antibody (BioLegend), or Fas Ligand (5 µg/mouse i.v.; R&D Systems). All antibodies were administered at 100 µg/mouse i.p. daily for 3 d.
To assess the effect of acute inflammation, thioglycolate (Sigma-Aldrich) was administered i.p. (3 ml of 3% solution/mouse once). To examine the effect of caspase inhibition, mice were treated with the pan-caspase inhibitor Z-VAD (OMe)-FMK (3 mg/kg IP daily for 5 d; Merck Millipore). Clophosome (200 µl/mouse; FormuMax Scientific Inc.) was administered i.p. to deplete macrophages.
To obtain chimeric mice, 6-wk-old WT mice were lethally irradiated at a dose of 9.5 Gy, after which they were transplanted with BM total cells from age-matched IL-1R−/−/CAG-eGFP, IL-1α−/−/CAG-eGFP, or control (IL-1R+/+/CAG-eGFP or IL-1α+/+/CAG-eGFP) mice. 6 wk after transplantation, BM dynamics were scored.
Nishimura S., Nagasaki M., Kunishima S., Sawaguchi A., Sakata A., Sakaguchi H., Ohmori T., Manabe I., Italiano JE J.r., Ryu T., Takayama N., Komuro I., Kadowaki T., Eto K, & Nagai R. (2015). IL-1α induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs. The Journal of Cell Biology, 209(3), 453-466.
+ Open protocol
+ Expand
4

SARS-CoV-2 Immune Response Stimulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were counted and resuspended in Dutch modified Roswell Park Memorial Institute (RPMI) medium (Life Technologies), supplemented with 50 µg/mL gentamicin (Thermo Fisher Scientific), 1 mM sodium pyruvate (Thermo Fisher Scientific), and 2 mM Glutamax (Thermo Fisher Scientific). 5 x 105 PBMCs were cultured in a final volume of 200 μL/well in round bottom 96-well plates (Greiner Bio-one) and stimulated with of heat-inactivated SARS-CoV-2 Wuhan-Hu-1 virus variant (1.19 x 104 TCID50/ml; NRW-42 isolate, kindly provided by Heiner Schaal, University Hospital Düsseldorf, Germany) (17 (link)), heat-inactivated Staphylococcus aureus (1 x 106 CFU/mL; clinical isolate), heat-inactivated influenza virus H1N1 (3.2 x 105 K/mL, kindly provided by Ortwin Adams, University Hospital Düsseldorf, Germany), Imiquimod/R837 (10 µg/ml; InvivoGen), Poly (I:C) (10 µg/mL; InvivoGen), R848 (10 µg/mL; InvivoGen), purified lipopolysaccharide (LPS) derived from Escherichia coli O55:B5 (10 ng/mL; Sigma-Aldrich), or recombinant human interleukin-1 alpha (IL-1α) (10 ng/mL; R&D Systems), in the presence of dexamethasone (10 nM or otherwise stated; Sigma-Aldrich) or vehicle control (dimethyl sulfoxide; DMSO). After 4h cells were lysed in RLT buffer (Qiagen) and stored at −80°C for subsequent RNA isolation. After 24 h or 7 days, supernatants were collected and stored at –80°C until analysis.
Engel J.J., van der Made C.I., Keur N., Setiabudiawan T., Röring R.J., Damoraki G., Dijkstra H., Lemmers H., Ioannou S., Poulakou G., van der Meer J.W., Giamarellos-Bourboulis E.J., Kumar V., van de Veerdonk F.L., Netea M.G, & Ziogas A. (2023). Dexamethasone attenuates interferon-related cytokine hyperresponsiveness in COVID-19 patients. Frontiers in Immunology, 14, 1233318.
+ Open protocol
+ Expand
5

Cytokine Stimulation Cocktail Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following cytokines were used at 10 ng/mL as M5 cocktail: IL-17A, IL-1α, Oncostatin M (all from R&D Systems), IL-22 (MACS Miltenyl Biotec), TNFα (PeproTech London, UK).
Shutova M.S., Borowczyk J., Russo B., Sellami S., Drukala J., Wolnicki M., Brembilla N.C., Kaya G., Ivanov A.I, & Boehncke W.H. (2023). Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2. iScience, 26(3), 106195.
+ Open protocol
+ Expand
6

Caspase Activation and Pyroptosis Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
LPS was purchased from Santa Cruz Biotechnology, nigericin, and vildagliptin and Ac-YVAD-CMK from the Cayman Chemical Company, PMA and sitagliptin from Sigma, Ala-Pro-AFC from Bachem, saxagliptin from Toronto Research Chemicals, and Z-VAD-FMK and etoposide from Enzo Life Sciences. Val-boroPro45 (link), 1G24424 (link), FP-biotin15 (link), L-allo-Ile-isoindoline14 (link), and L-allo-Ile-thiazolidine14 (link) were synthesized according to previously published protocols. For cell culture experiments, Val-boroPro was resuspended in DMSO containing 0.1% TFA to prevent compound cyclization. Antibodies used include: human caspase-1 (#2225, Cell Signaling Technology), mouse caspase-1 (clone Casper-1, Adipogen), caspase-3 (clone 8G10, Cell Signaling Technology), human caspase-4 (clone 4B9, Santa Cruz), human caspase-5 (clone D3G4W, Cell Signaling Technology), caspase-7 (clone D2Q3L, Cell Signaling Technology), human IL-1β (Clone 2805, R&D Systems), mouse IL-1β (clone D4T2D, Cell Signaling Technology), IL-1α (#AF-200, R&D Systems), IL-18 (#AF2548, R&D Systems), GAPDH (clone 14C10, Cell Signaling Technology), DPP7 (Clone 398024, R&D Systems), DPP8 (ab42076, Abcam), DPP9 (ab42080, Abcam), PARP (#9542, Cell Signaling Technology), GSDMD (NBP2-33422, Novus Biologicals), DPP4 (#11D7, GeneTex), FAP (ABT11, Millipore), and SCPEP1 (SAB2700267, Sigma).
Okondo M.C., Johnson D.C., Sridharan R., Go E.B., Chui A.J., Wang M.S., Poplawski S.E., Wu W., Liu Y., Lai J.H., Sanford D.G., Arciprete M.O., Golub T.R., Bachovchin W.W, & Bachovchin D.A. (2016). DPP8/9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Nature chemical biology, 13(1), 46-53.
+ Open protocol
+ Expand
7

Plasma Cytokine Profiling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma cytokines were measured using a Bioplex multiplex cytokine assay system (Bio-Rad, Hercules, CA). The parameters analyzed were IFNγ, TNFα, IL-2, IL-17A, IL-4, IL-5, IL-10, IL-6, IL-12p70 and GM-CSF. Plasma levels of TGFβ, IL-1α, IL-1β (all R& D Systems); IL-17F (Biolegend); IL-22 (eBioscience); Type 1 interferons (IFNs) - IFNα (multiple subtypes) and IFNβ (PBL Interferon Source) were measured by ELISA. All samples were run in duplicates.
George P.J., Kumar N.P., Jaganathan J., Dolla C., Kumaran P., Nair D., Banurekha V.V., Shen K., Nutman T.B, & Babu S. (2015). Modulation of pro- and anti - inflammatory cytokines in active and latent tuberculosis by coexistent Strongyloides stercoralis infection. Tuberculosis (Edinburgh, Scotland), 95(6), 822-828.
+ Open protocol
+ Expand
8

Isolation and Characterization of Immune Cells in Rheumatoid Arthritis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
NL and RA peripheral blood (PB) and RA SF mononuclear cells were isolated by Ficoll-Paque gradient centrifugation as described previously 18 -22 (link). Monocytes were isolated from NL and RA PB using a negative selection kit, according to the manufacturer’s instructions 18 -22 (link). Monocytes were subsequently differentiated to macrophages by culturing in 20% FBS containing media 18 -22 (link). EPCs were isolated from NL blood. Briefly, NL peripheral blood mononuclear cells (PBMC)s were isolated and cultured on a collagen coated plate in 10% FBS containing EBM2 media. After 1 day, nonadherent cells were discarded and media was replaced daily for 2-4 weeks. Subsequently, endothelial colonies were isolated by a colony ring and were transferred to a new plate where only a homogenous population of endothelial cells was growing 23 (link).
RA PB monocytes, RA PB in vitro differentiated macrophages and human umbilical vein endothelial cells (HUVECs) (Lonza, Walkersville, MD) were either untreated or treated with 10 ng/ml LPS (Invivogen, San Diego, CA), TNF-α, IL-1α, IL-6 or 50 ng/ml IL-17 (R&D Systems, Minneapolis, MN) for 24-72h prior to detecting CCL28 production by ELISA or CCR10 expression via FACS analysis (1:1000; Biolegend) or Western blotting (1:1000; Santa Cruz Biotechnology).
Chen Z., Kim S.J., Essani A.B., Volin M.V., Vila O.M., Swedler W., Arami S., Volkov S., Sardin L.V., Sweiss N, & Shahrara S. (2014). Characterizing the expression and function of CCL28 and its corresponding receptor CCR10 in RA pathogenesis. Annals of the rheumatic diseases, 74(10), 1898-1906.
+ Open protocol
+ Expand
9

Isolation and Culture of Trophoblast Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Placental tissues were obtained from born placentas of healthy pregnancies and from clinically normal human pregnancies, which were interrupted for psychosocial reasons (approved by the Ethical Committee at the Medical University of Graz [23-203 ex 10/11] and the Friedrich-Schiller-University Jena [1503-03/05]). Mononucleated human first trimester and term trophoblast cells were isolated as described [15 (link),16 (link)]. Outgrowing EVT cells were isolated from placental villi after terminations of pregnancy (7–10 weeks, n = 5) and were incubated in Petri dishes for two days at 5% CO2 in DMEM/Ham’s F12 with 10% (v/v) human serum. Then the villi were transferred into collagen-coated 24-well plates (two explants/well) and incubated at 2.5% O2, 5% CO2. After three days of culture the villi were removed from the plates and the already outgrown cells were cultured for another day at 5% CO2. Human hepatocellular carcinoma (HUH-7) cells, human Jeg-3 and human AC1-M59 cells were cultured as described [12 (link),17 ,18 ]. AC1-M59 and Jeg-3 cells were seeded into cell culture dishes and upon reaching 80% confluence, incubated for 24 h in medium containing 10 ng/ml IL-1α, IL-1β, IL-6, or TNFα (R&D Systems, Minneapolis, MN, USA).
Rossmann C., Hammer A., Koyani C.N., Kovacevic A., Siwetz M., Desoye G., Poehlmann T.G., Markert U.R., Huppertz B., Sattler W, & Malle E. (2014). Expression of serum amyloid A4 in human trophoblast-like choriocarcinoma cell lines and human first trimester/term trophoblast cells. Placenta, 35(8), 661-664.
+ Open protocol
+ Expand
10

Pro-inflammatory Cytokine Response in HMEEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human ME epithelial cells (HMEEC) were provided by Dr. David Lim (House Ear Institute). Normal growth media consisted of 1:1 mixture of Bronchial Epithelial Cell Growth Medium (BEBM; Cambrex, East Rutherford, NJ) and Dulbecco’s Modified Eagle Medium (DMEM) supplemented with final concentrations of 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (ThermoFisher, Carlsbad, CA). Cells were grown in a humidified chamber at 37°C with 5% carbon dioxide and media changed every three days. Cells were grown to 70–80% confluency and serum-starved for two hours prior to exposure to pro-inflammatory cytokines: 200ng/ml tumor necrosis factor-alpha (TNF-α) or 100ng/ml interleukin 1 beta (IL-1α; R&D Systems, Minneapolis, MN) or normal growth media alone (control) for 2, 4, 6, 8, 16 and 24 hours, harvested and stored at −80C. All treatment conditions and time points were performed in triplicate.
Samuels T.L., Yan J., Khampang P., MacKinnon A., Hong W., Johnston N, & Kerschner J.E. (2016). Association of microRNA 146 with middle ear hyperplasia in pediatric otitis media. International journal of pediatric otorhinolaryngology, 88, 104-108.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!