Anti ha

Manufactured by Medical & Biological Laboratories

Sourced in Japan

Anti-HA is a laboratory reagent used for the detection and purification of proteins tagged with the Hemagglutinin (HA) epitope. It binds specifically to the HA tag, allowing for the identification and isolation of HA-tagged proteins from complex samples.

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Lab products found in correlation

Anti myc, by Medical & Biological Laboratories (3 mentions) Anti flag, by Medical & Biological Laboratories (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Anti gapdh 5a12, by Fujifilm (2 mentions) Monoclonal anti myc antibody 9b11, by Cell Signaling Technology (2 mentions) Anti ddddk, by Medical & Biological Laboratories (2 mentions) Anti flag m2 monoclonal antibody, by Merck Group (2 mentions) Ab80588, by Abcam (1 mentions) Ab32049, by Abcam (1 mentions) Ab68477, by Abcam (1 mentions) Ab109012, by Abcam (1 mentions) Anti ubiquitin, by Proteintech (1 mentions) Anti his, by Medical & Biological Laboratories (1 mentions) Anti flag, by Proteintech (1 mentions) Anti slc7a11, by Novus Biologicals (1 mentions) Anti slc7a11, by Proteintech (1 mentions) Nutlin 3, by Selleck Chemicals (1 mentions) Erastin, by Selleck Chemicals (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti p53, by Proteintech (1 mentions)

6 protocols using anti ha

Western Blot Analysis of Cellular Proteins

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The antibodies included the following: anti-SQSTM1/p62 (M162-3, Medical Biological Laboratories, Japan), anti-SQSTM1/p62 (ab109012, Abcam, USA), anti-p53 (sc-126, Santa Cruze Biotechnology, USA), anti-p53 (10,442–1-AP, Proteintech, China), anti-mutant p53 (ab32049, Abcam), anti-NRF2 (M200-3, Medical Biological Laboratories), anti-NRF2 (16,396–1-AP, Proteintech), anti-ubiquitin (10,201–2-AP, Proteintech), anti-GAPDH (#5174, Cell Signaling Technology), anti-β-actin (#4970, Cell Signaling Technology), anti-HA (M180-3 and M561, Medical Biological Laboratories), anti-His (D291-3, Medical Biological Laboratories), anti-Flag (M185, Medical Biological Laboratories), anti-Flag (20,543–1-AP, Proteintech), anti-SLC7A11 (NB300-318, Novus, USA), anti-SLC7A11 (26,864–1-AP, Proteintech) anti-HO1 (ab68477, Abcam), anti-HO1 (10,701–1-AP, Proteintech), anti-NQO1 (ab80588, Abcam), anti-Keap1 (10,503–2-AP, Proteintech).
The reagents included the following: Erastin (S7242, Selleck, USA), APR-246 (HY-19980, MCE, USA), Pifithrin-α (HY-15484, MCE), Nutlin-3 (S1061, Selleck).

Yuan F., Sun Q., Zhang S., Ye L., Xu Y., Deng G., Xu Z., Zhang S., Liu B, & Chen Q. (2022). The dual role of p62 in ferroptosis of glioblastoma according to p53 status. Cell & Bioscience, 12, 20.

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Antibody Characterization and Detection

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Polyclonal antibodies, Anti-DDDDK, anti-Myc and anti-HA were obtained from Medical and Biological Laboratories (Japan). Monoclonal antibody, anti-GAPDH (5A12), was purchased from Wako (Japan). Anti-SPAK/OSR1 and anti-phospho-SPAK/OSR1 antibodies were prepared as previously described^{6 (link)}. These antibodies were used for immunoblotting at 1/1000. anti-Myc (9B11) monoclonal antibody was purchased from Cell Signaling Technology (Danvers, MA, USA) and anti-Flag (M2) monoclonal antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA), and these antibodies were used for immunoprecipitation at 1/500.

Shimizu M, & Shibuya H. (2022). WNK1/HSN2 mediates neurite outgrowth and differentiation via a OSR1/GSK3β-LHX8 pathway. Scientific Reports, 12, 15858.

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Western Blot Analysis of Protein Modifications

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Samples were heat-denatured and separated via SDS-PAGE, followed by transfer to polyvinylidene fluoride membranes (Immobilon-P) (Merck Millipore, Watford, UK). Membranes were blocked with Blocking One (Nacalai Tesque) or Tris-buffered saline-Tween (TTBS) containing 3% skim milk (Nacalai Tesque), after which they were incubated with antibodies in TTBS containing 5% skim milk overnight at 4°C. Antibodies used in this study included the following: anti-DDDDK (FLAG)-tag (Medical & Biological Laboratories Co., Ltd., Nagoya, Japan), anti–β-actin (Santa Cruz Biotechnology, Santa Cruz, CA), anti-ADH5 prepared as we described elsewhere (29 (link)), anti-protein S-glutathionylation (Virogen, Watertown, MA), anti-yeast 3-phosphoglycerate kinase (Pgk1) (clone: 22C5D8; Abcam, Cambridge, UK), and anti-HA (Medical & Biological Laboratories Co., Ltd.). Membranes were washed three times with TTBS and then incubated with a horseradish peroxidase–conjugated secondary antibody for 1 hour at room temperature. After the membranes were washed again three times with TTBS, immunoreactive bands were detected via a chemiluminescence reagent (ECL Prime Western Blotting Detection Reagent; GE Healthcare) with a luminescent image analyzer (ImageQuant LAS 500; GE Healthcare).

Kasamatsu S., Nishimura A., Alam M.M., Morita M., Shimoda K., Matsunaga T., Jung M., Ogata S., Barayeu U., Ida T., Nishida M., Nishimura A., Motohashi H, & Akaike T. (2023). Supersulfide catalysis for nitric oxide and aldehyde metabolism. Science Advances, 9(33), eadg8631.

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Antibody Characterization Protocol

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Anti‐USP11 (catalog no. sc‐365528), anti‐Ub (catalog no. sc‐166553) and anti‐NONO (catalog no. sc‐376865) antibodies were purchased from Santa Cruz. Anti‐USP11 (EPR4346) and anti‐Myc (2276S) antibodies were provided by Abcam. Three antibodies against NONO were obtained from Sangon Biotech (catalog no. D199144), MBL Life science (catalog no. RN092PW), Proteintech Group, Inc (catalog no. 11058‐1‐AP). Anti‐Flag (catalog no. M185‐3L), anti‐HA (catalog no. M180‐3) and anti‐Myc (catalog no.M192‐3) were purchased from Medical & Biological Laboratories. Anti‐GAPDH (catalog no. KC‐5G4) antibody was bought from Kangchen Biotech.

Feng P., Li L., Dai J., Zhou L., Liu J., Zhao J., Li X., Ling N., Qiu S., Zhang L., Xie T., Chen Y., Donovan M.J., Peng T., Song J, & Ye M. (2020). The regulation of NONO by USP11 via deubiquitination is linked to the proliferation of melanoma cells. Journal of Cellular and Molecular Medicine, 25(3), 1507-1517.

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Western Blot Antibody Validation

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Western blotting assay was performed as previously described^{46 (link)}. The anti-NHERF1 was purchased from Sigma-Aldrich (HPA009672, St. Louis, MO) and Becton Dickinson Labware (#611161, Billerica, MA), respectively. Anti-HA (#561) was purchased from Medical & Biological Laboratories (Nagoya, Japan). Anti-c-Myc (#ab32072) and anti-β-catenin (#ab22656) were purchased from Abcam (Cambridge, UK). Anti-GAPDH (#5174), anti-β-catenin (#9581), and anti-TCF-1 (#2206) were purchased from Cell Signaling Technology (Danvers, MA). Anti-ACTN4 was purchased from Enzo Life Sciences (#ALX-210-356, Shanghai, China) and Santa Cruz Biotechnology (#sc-134236, Santa Cruz, CA), respectively. Anti-Ki67 (#zm-0166) and horse radish peroxidase-conjugated secondary antibodies were purchased from ZSGB-BIO (Beijing, China). Infrared fluorescent dyes-conjugated secondary antibodies were purchased from LI-COR Biosciences (Lincoln, NE). IWR-1-endo (#S7086) was purchased from Selleck (Houston, TX).

Wang Q., Qin Q., Song R., Zhao C., Liu H., Yang Y., Gu S., Zhou D, & He J. (2018). NHERF1 inhibits beta-catenin-mediated proliferation of cervical cancer cells through suppression of alpha-actinin-4 expression. Cell Death & Disease, 9(6), 668.

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Antibody sourcing and use for immunoblotting and immunoprecipitation

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Polyclonal antibodies, Anti-DDDDK, anti-Myc and anti-HA were obtained from Medical & Biological Laboratories (Japan). Monoclonal antibody, anti-GAPDH (5A12), was purchased from Wako (Japan).
Anti-OSR1 and anti-phospho-OSR1 antibodies were prepared as previously described 6 . These antibodies were used for immunoblotting at 1/1000. anti-Myc (9B11) monoclonal antibody was purchased from Cell Signaling Technology (Danvers, MA, USA) and anti-Flag (M2) monoclonal antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA), and these antibodies were used for immunoprecipitation at 1/500.

Shimizu M., & Shibuyà H. (2022). WNK1/HSN2 Regulates Neural Development via GSK3β.

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