Eclipse 90i upright

Manufactured by Nikon

The Eclipse 90i Upright is a high-performance microscope system designed for advanced imaging applications. It features a stable and ergonomic design, providing a reliable and consistent platform for research and analysis.

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Lab products found in correlation

Coolsnap hq2, by Teledyne (3 mentions) Piezoelectric motor, by Physik Instrumente (2 mentions) Tcs sp5 2, by Leica (1 mentions) Las af, by Leica (1 mentions) Application suite advanced fluorescence, by Leica (1 mentions) 4 6 diamino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Nis element software, by Nikon (1 mentions) Ds qi1mc, by Nikon (1 mentions)

Close spelling variants of this product

Eclipse 90i (564 citations) ECLIPSE 90i upright microscope (23 citations)

8 protocols using eclipse 90i upright

Microscopy Techniques for Biological Analysis

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Immunohistochemical preparations were analyzed with a Leica TCS SP5 II confocal laser-scanning microscope equipped with DPSS-, Diode- and Argon-lasers and operated by the Leica Application Suite Advanced Fluorescence software package (LASAF). Images of single frames and maximal projections were compiled using the software LASAF (Leica Microsystems CMS GmbH) and image processing platform Fiji [51 (link)].
Specimens processed for histology were analyzed with a Nikon Eclipse 90i upright microscope and bright-field optics (20x objective).
The precopula was photographed with a Canon 70d camera equipped with an EF-S 18–135 mm f/3.5–5.6 IS objective and a Macro Twin Lite MT-24EX flashlight. Cross-polarized light was used to minimize reflections [52 ].

Wittfoth C., Harzsch S., Wolff C, & Sombke A. (2019). The “amphi”-brains of amphipods: new insights from the neuroanatomy of Parhyale hawaiensis (Dana, 1853). Frontiers in Zoology, 16, 30.

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Multimodal Immunostaining of Neural Cultures

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Cultures were fixed by incubation with 4% paraformaldehyde in PBS for 10 minutes at room temperature. Cells were permeabilized by incubation with 0.5% Triton X-100 for 5 minutes at room temperature before blocking with 30 mg/mL bovine serum albumin (BSA, Sigma) in PBS. Cultures were first immunostained with an antibody against NC1 (mouse IgM, produced in-house, diluted to 10 μg/mL) and a Cy3-labeled donkey anti-mouse IgM secondary antibody (Jackson, diluted 1/300). Cultures were then blocked by incubation with control anti-mouse IgG for 20 minutes in the dark at room temperature and immunolabeled with Alexa Fluor 488-labeled mouse anti-TUJ1 (class III beta-tubulin) antibody (Covence, diluted 1/500) and with 4′,6-diamino-2-phenylindole (DAPI, Molecular Probes, diluted 1/1000). Fluorescence image acquisition was performed on an epifluorescence-histology microscope (Eclipse 90i Upright) at the Nikon Imaging Centre @ Institut Curie-CNRS. This microscope was equipped with a CCD Camera (CoolSNAP HQ2, Photometrics) and piloted with MetaMorph software. Entire explant images were reconstituted with Photoshop software, with the adjustment of brightness and contrast over the entire image to enhance fluorescence signals.

Chevalier N.R., Gazguez E., Bidault L., Guilbert T., Vias C., Vian E., Watanabe Y., Muller L., Germain S., Bondurand N., Dufour S, & Fleury V. (2016). How Tissue Mechanical Properties Affect Enteric Neural Crest Cell Migration. Scientific Reports, 6, 20927.

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Quantifying Motor Protein-Endosome Associations

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For 3D deconvolution analysis, images were acquired with a wide-field microscope (Eclipse 90i Upright; Nikon) using a 100× Plan Apo VC 1.4 oil objective and a highly sensitive cooled interlined charge-coupled device (CCD) camera (CoolSNAP HQ2; Roper Scientific). A z-dimension series of images was taken every 0.2 µm by means of a piezoelectric motor (Physik Instrumente), and images were deconvoluted (Sibarita, 2005 (link)). To quantify the association of motor proteins to MT1-MMP endosomes, a motor association index was calculated based on segmentation of MT1-MMPmCh–positive endosomes by the thresholding of deconvoluted images. Then, the number of dots of motor proteins associated with each segmented object (MT1-MMP–positive endosomes) was scored using a “particle counting inside regions of interest” macro in ImageJ. The number of dots was normalized to the total number of dots for each motor protein per cell (Motor Association Index) by setting the index of siNT-treated cells to 100.

Marchesin V., Castro-Castro A., Lodillinsky C., Castagnino A., Cyrta J., Bonsang-Kitzis H., Fuhrmann L., Irondelle M., Infante E., Montagnac G., Reyal F., Vincent-Salomon A, & Chavrier P. (2015). ARF6–JIP3/4 regulate endosomal tubules for MT1-MMP exocytosis in cancer invasion. The Journal of Cell Biology, 211(2), 339-358.

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Quantification of Invadopodia-Associated Proteins

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5 × 10⁴ cells were plated on type I collagen–coated coverslips, fixed after 60 min, and stained with TKS5 or FGD1 pAbs and counterstained with cortactin mAb. Images were acquired with a wide-field microscope (Eclipse 90i Upright; Nikon) using a 100× Plan Apo VC 1.4 oil objective and a highly sensitive cooled interlined charge-coupled device camera (CoolSNAP HQ2; Roper Scientific). A z-dimension series of images was taken every 0.2 µm by means of a piezoelectric motor (Physik Instrumente). For quantification of TKS5 or FGD1 associated with invadopodia, three consecutive z-planes corresponding to the plasma membrane in contact with collagen fibers were projected using maximal-intensity projection in Fiji, and TKS5 or FGD1 signal was determined using the thresholding command excluding regions <8 px to avoid noninvadopodial structures. Surface covered by TKS5 or FGD1 signal was normalized to the total cell surface, and values were normalized to those of control cells.

Zagryazhskaya-Masson A., Monteiro P., Macé A.S., Castagnino A., Ferrari R., Infante E., Duperray-Susini A., Dingli F., Lanyi A., Loew D., Génot E, & Chavrier P. (2020). Intersection of TKS5 and FGD1/CDC42 signaling cascades directs the formation of invadopodia. The Journal of Cell Biology, 219(9), e201910132.

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Quantification of Invadopodia-associated Tks5

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5 × 10⁴ cells were plated on collagen-coated coverslips, fixed after 60 min and stained with Tks5 and cortactin antibodies. Images were acquired with a wide-field microscope (Eclipse 90i Upright; Nikon) using a 100 × Plan Apo VC 1.4 oil objective and a highly sensitive cooled interlined charge-coupled device (CCD) camera (CoolSnap HQ2; Roper Scientific). A z-dimension series of images was taken every 0.2 µm by means of a piezoelectric motor (Physik Instrumente). For quantification of Tks5 associated with invadopodia, three consecutive z-planes corresponding to the plasma membrane in contact with collagen fibers were projected using maximal intensity projection in Fiji and Tks5 signal was determined using the thresholding command excluding regions < 8 px to avoid non-invadopodial structures. Surface covered by Tks5 signal was normalized to the total cell surface and values normalized to that of control cells.

Ferrari R., Martin G., Tagit O., Guichard A., Cambi A., Voituriez R., Vassilopoulos S, & Chavrier P. (2019). MT1-MMP directs force-producing proteolytic contacts that drive tumor cell invasion. Nature Communications, 10, 4886.

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Microscopic Imaging and Image Processing

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We imaged tissue on an upright microscope (Nikon Eclipse 90i upright) with a 4×, 10× and 40× objectives. NIS Elements Advanced Research v3.10 imaging software was used to obtain and to analyze images. Enhancement was limited to program algorithms, applied evenly to all samples, and only included deconvolution and sharpening with Gauss-LaPlace. To capture whole mount embryo images, we used a SMZ1000 Nikon microscope with both Fiber Optic Gooseneck and Ring Light sources and NIS-Elements Software 4.11. We used Adobe Photoshop Elements v9.0 to assemble and produce the figures.

Kousa Y.A., Moussa D, & Schutte B.C. (2017). IRF6 expression in basal epithelium partially rescues Irf6 knockout mice. Developmental dynamics : an official publication of the American Association of Anatomists, 246(9), 670-681.

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Transferrin Receptor Trafficking in Dendritic Cells

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In all, 3 × 10⁵ DCs were loaded in microchannels 10 h before the pulse-chase. First, transferrin receptors (CD71) expressed at the surface were stained at 4 °C with antibodies diluted in medium for 20 min. After three washes with medium at 4 °C, DCs were incubated at 37 °C for 30 min, and then fixed inside micro-channels. Immunofluorescence was then performed as described above (see Immunofluorescence section) using phalloïdin-AF546, Hoechst and endosomal markers: anti-ClathrinHC, anti-EEA1, anti-Rab11, anti-Rab7, anti-Lamp1 and phalloïdin. Images were acquired on an epifluorescence Nikon microscope (Eclipse 90i Upright) with a × 100 objective. Deconvolution was performed on stacks of images taken with a 100-nm z section, using the 3D deconvolution module of METAMORPH and the fast iterative constrained point spread function-based algorithm47 . Quantifications were performed using Image J. Phalloïdin staining was used as a mask for the cell. For endosomal and CD71 staining, foci were detected by applying a threshold and object segmentation by maxima.

Chabaud M., Heuzé M.L., Bretou M., Vargas P., Maiuri P., Solanes P., Maurin M., Terriac E., Le Berre M., Lankar D., Piolot T., Adelstein R.S., Zhang Y., Sixt M., Jacobelli J., Bénichou O., Voituriez R., Piel M, & Lennon-Duménil A.M. (2015). Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nature Communications, 6, 7526.

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Histological Analysis of Murine Exorbital and Submandibular Glands

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The day after collecting tear and saliva, the mice were humanely euthanized and their submandibular gland (SMG) and exorbital lacrimal gland (ELG) were excised, fixed in 10% formalin for 24 h and paraffin-embedded. The tissue sections (4 µm) were routine-stained with by H and E. Using a Nikon Eclipse 90i upright microscope fitted with a Nikon DS-Qi1Mc camera, the H and E stained sections were photo-imaged with a 4X objective lens. The number of lymphocytic infiltrate foci (defined as an aggregate of >50 monocytes) were counted in each SMG or ELG section. To determine the percent infiltrate area, the infiltrate areas and the total tissue section area of each image were measured using Nikon NIS-Element software. The data are presented as the mean percentage of infiltrate area in the SMG or ELG sections. One SMG or ELG tissue section was analyzed per mouse. All slides were coded with ID numbers and the histological assessments were performed in a blinded manner.

Song M., Tian J., Middleton B., Nguyen C.Q, & Kaufman D.L. (2022). GABA Administration Ameliorates Sjogren’s Syndrome in Two Different Mouse Models. Biomedicines, 10(1), 129.

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