Ox40 apc

Manufactured by BioLegend

Sourced in United States

OX40-APC is a fluorochrome-conjugated antibody that binds to the OX40 protein, also known as TNFRSF4 or CD134. OX40 is a member of the tumor necrosis factor receptor superfamily and is expressed on activated T cells. The APC (Allophycocyanin) fluorochrome is attached to the antibody, enabling detection and analysis of OX40-expressing cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Ccr7 pe, by BioLegend (2 mentions) Cd3 apc a750, by BioLegend (2 mentions) Cd4 pb450, by BioLegend (2 mentions) Cd8a apc a700, by BioLegend (2 mentions) Cd69 pc7, by BioLegend (2 mentions) 7aad solution, by Beckman Coulter (2 mentions) Cytoflex s, by Beckman Coulter (2 mentions) Cd45ra fitc, by BioLegend (1 mentions) Cd3 percp cy5, by BioLegend (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Fix perm buffer, by Thermo Fisher Scientific (1 mentions) Cd8a pe cy7, by BioLegend (1 mentions) Cd62l apc, by BioLegend (1 mentions) Cd44 pe, by BioLegend (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions) Pd 1 fitc, by Thermo Fisher Scientific (1 mentions) Ccr7 bv421, by BioLegend (1 mentions) Ifnγ bv421, by BioLegend (1 mentions)

3 protocols using ox40 apc

SARS-CoV-2-Specific T Cell Activation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Activation Induced marker assay was performed as reported earlier^{3 (link)}. Briefly, PBMCs (0.7 million cells/100 µl) were plated onto separate 96 well U bottom plates and stimulated with the cocktail of SARS-CoV-2 peptide pool (1 µg/mL). After overnight stimulation, cells were washed and stained 40 minutes with antibody cocktail containing the following fluorescently conjugated antibodies obtained from Biolegend, USA: CD3-APC-A750 (Cat. 300470), CD4-PB450 (Cat. 300521), CD8a-APC-A700 (Cat. 301028), OX40-APC (Cat. 350008), CD137-ECD (Cat. 309826), CD69-PC7 (Cat. 310912), CCR7-PE (Cat. 353204), CD45RA-FITC (Cat. 304148). The plates were centrifuged and the resuspended cells were labelled again with 7AAD solution (Cat. 6604104, Beckman) and analysed in a flow cytometer (CytoFlex S, Beckman Coulter).

Singh C., Verma S., Reddy P., Diamond M.S., Curiel D.T., Patel C., Jain M.K., Redkar S.V., Bhate A.S., Gundappa V., Konatham R., Toppo L., Joshi A.C., Kushwaha J.S., Singh A.P., Bawankule S., Ella R., Prasad S., Ganneru B., Chiteti S.R., Kataram S, & Vadrevu K.M. (2023). Phase III Pivotal comparative clinical trial of intranasal (iNCOVACC) and intramuscular COVID 19 vaccine (Covaxin®). NPJ Vaccines, 8, 125.

+ Open protocol

+ Expand

Murine Liver TIL Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

After isolation of the TILs from the liver, 100 μL volumes of cell suspension at a concentration of 1×10⁷ cells/mL were plated into designated wells of a 96-well plate. Cells were first stained with Live-Dead Aqua (Invitrogen) for 30 minutes on ice, washed twice with PBS, and then blocked with rat anti-mouse Fc antibody (CD16/CD32, BD Biosciences). After blocking, cells were incubated for 1 hour with the following anti-mouse fluorophores: CD3-PerCP-Cy5.5 (Biolegend), CD4-APC-Fire (BD Biosciences), CD8a-PE/Cy7 (Biolegend), PD-1-FITC (eBioscience), OX40-APC (Biolegend), CD44-PE (Biolegend), CD62L-APC (Biolegend), and CCR7-BV421 (Biolegend). Cells were washed following staining, and suspended in Fix/Perm buffer (eBioscience) for 30 minutes. After fixation, intracellular staining with Eomes PE (Invitrogen) and IFNγ BV421 (Biolegend) was done on ice for 30 minutes. Flow cytometry was performed using the CytoFLEX flow cytometer (B eckman Coulter).

Muth S.T., Saung M.T., Blair A.B., Henderson M.G., Thomas DL I.I, & Zheng L. (2020). CD137 Agonist-based Combination Immunotherapy Enhances Activated, Effector Memory T cells and Prolongs Survival in Pancreatic Adenocarcinoma. Cancer letters, 499, 99-108.

+ Open protocol

+ Expand

SARS-CoV-2 Specific T Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Activation Induced marker assay was performed as reported earlier³⁰. Briefly, PBMCs (0.7 million cells/100 µl) were plated onto separate 96 well U bottom plates and stimulated with the cocktail of SARS-CoV-2 peptide pool (1 µg/mL). After overnight stimulation, cells were washed and stained 40 min with antibody cocktail containing the following fluorescently conjugated antibodies obtained from Biolegend, USA: CD3-APC-A750 (300470), CD4-PB450 (300521), CD8a-APC-A700 (301028), OX40-APC (350008), CD137-ECD (309826), CD69-PC7 (310912), CCR7-PE (353204), CD45RA-FITC (304148). The plates were centrifuged and the resuspended cells were labelled again with 7AAD solution (6604104, Beckman) and analysed in a flow cytometer (CytoFlex S, Beckman Coulter).

Vadrevu K.M., Ganneru B., Reddy S., Jogdand H., Raju D., Sapkal G., Yadav P., Reddy P., Verma S., Singh C., Redkar S.V., Gillurkar C.S., Kushwaha J.S., Mohapatra S., Bhate A., Rai S.K., Ella R., Abraham P., Prasad S, & Ella K. (2022). Persistence of immunity and impact of third dose of inactivated COVID-19 vaccine against emerging variants. Scientific Reports, 12, 12038.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!