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Wako labassay cholesterol kit

Manufactured by Fujifilm
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Sourced in Germany

The Wako LabAssay Cholesterol kit is a laboratory reagent used for the quantitative determination of total cholesterol levels in biological samples. It provides a reliable and accurate method for measuring cholesterol concentrations.

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Lab products found in correlation

Citrate assay kit, by Merck Group (1 mentions) Lactate assay kit, by Merck Group (1 mentions) Wako labassay triglyceride kit, by Fujifilm (1 mentions) Tissuelyser 2, by Qiagen (1 mentions)

2 protocols using wako labassay cholesterol kit

1

Hepatic Lipid and Metabolite Analysis

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Flash frozen liver tissue (50 mg) was homogenized in a TissueLyser II (Qiagen Cat. No./ID: 85300). For TAG and cholesterol assays, liver homogenate was prepared as previously described.[25] The dried sample was resuspended in water before TAG and cholesterol analysis. Hepatic TAG (Wako LabAssay Triglyceride kit, Fuggerstraße, Neuss, Germany) and hepatic cholesterol (Wako LabAssay Cholesterol kit) was measured as per manufacturer's instructions. Hepatic citrate (Sigma‐Aldrich Citrate Assay Kit) and lactate (Sigma‐Aldrich Lactate Assay Kit) were measured as per manufacturer's instructions.
Mitchelson K.A., Tran T.T., Dillon E.T., Vlckova K., Harrison S.M., Ntemiri A., Cunningham K., Gibson I., Finucane F.M., O'Connor E.M., Roche H.M, & O'Toole P.W. (2022). Yeast β‐Glucan Improves Insulin Sensitivity and Hepatic Lipid Metabolism in Mice Humanized with Obese Type 2 Diabetic Gut Microbiota. Molecular Nutrition & Food Research, 66(22), 2100819.
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2

Cholesterol Concentration Assessment

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Plasma samples were analyzed for the total cholesterol concentration (using the WAKO LabAssay Cholesterol Kit, Wako, Germany), according to the manufacturer’s instructions and absorbance was detected at 600 nm using a Sunrise microwell plate reader (Tecan, Switzerland).
Galabova G., Brunner S., Winsauer G., Juno C., Wanko B., Mairhofer A., Lührs P., Schneeberger A., von Bonin A., Mattner F., Schmidt W, & Staffler G. (2014). Peptide-Based Anti-PCSK9 Vaccines - An Approach for Long-Term LDLc Management. PLoS ONE, 9(12), e114469.
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