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Tissue lysate

Manufactured by Absin
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Sourced in China

Tissue lysate is a solution containing the cellular contents of a tissue sample. It is commonly used in research applications to extract and analyze proteins, nucleic acids, and other biomolecules from tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

Abs9225, by Absin (1 mentions) Luminex 200 system, by Thermo Fisher Scientific (1 mentions)

2 protocols using tissue lysate

1

CCL2 Cytokine Detection in Tumor Tissues

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At 24 h post administration, mice were bled for sera and killed for tumor collection. The obtained tumor was added to tissue lysate (absin, abs9225, Shanghai, China) at 10 mg tumor tissue/50 μL tissue lysate and homogenized using a tissue homogenizer. The supernatant was collected by centrifugation. Both sera and tumor tissue lysates were collected for CCL2 cytokine detection by mouse CCL2 ELISA kits (absin, abs520016).
Wu L., Li L., Li S., Liu L., Xin W., Li C., Yin X., Xu X., Bao F, & Hua Z. (2022). Macrophage-mediated tumor-targeted delivery of engineered Salmonella typhimurium VNP20009 in anti-PD1 therapy against melanoma. Acta Pharmaceutica Sinica. B, 12(10), 3952-3971.
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2

Cytokine Profiling in Murine Retinas

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Protein was extracted from the retinas from mice in each group at P17 with tissue lysate (Absin, Shanghai, China) containing protease inhibitors. The production of 8 cytokines (TNF-α, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-10, CXCL1) in retinal supernatants from each group was measured using the Mouse Cytokine Assay Kit (BIO-RAD, Richmond, VA, USA) according to the manufacturer’s instructions. The signals were detected and data analyzed by the Luminex 200 System (Thermo Scientific, San Diego, CA, USA).
Hong Y., Wang Y., Cui Y., Pan J., Mao S., Zhu Y., Wen T., Qi T., Wang A, & Luo Y. (2023). MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway. International Journal of Molecular Sciences, 24(14), 11767.
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