Bile salts b8631

AFB1 standard solution (purity > 99%) was acquired from Sigma-Aldrich (St. Louis, MO, USA). Potassium thiocyanate (KSCN), potassium chloride (KCl), hydrochloric acid (HCl), sodium dihydrogen phosphate (NaH₂PO₄), sodium hydroxide (NaOH), sodium sulfate (Na₂SO₄), sodium chloride (NaCl), sodium hydrogen carbonate (Na-HCO₃), urea (CO(NH₂)₂), pepsin A (674 U mg⁻¹ P7000), pancreatin (762 U mg⁻¹ P1750), bile salts (B8631), and α-amylase (930 U mg⁻¹ A3403) were purchased from Sigma-Aldrich (Madrid, Spain). Acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Madrid, Spain). Deionized water was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA). Dimethyl sulfoxide (DMSO) was acquired from Fisher Scientific (Geel, Belgium). Bread ingredients were purchased at a local supermarket. Fresh garlic was preserved at 4 °C and minced before use. Propidium iodide (PI) and a cycleTEST™ PLUS DNA Reagent Kit were obtained from BD Biosciences (San Diego, CA, USA) for the cell cycle assay by flow cytometry. The Annexin V–FITC conjugate was acquired from Miltenyi and the MitoTracker^® probe was purchased from Invitrogen (Waltham, MA, USA). 2′,7′-dichlorodihydrofluorescein diacetate (H₂-DCFDA) and phosphate buffer saline (PBS) were bought from Sigma Chemical Co. (St. Louis, MO, USA).

AFB1 and OTA standard solutions (purity > 99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium chloride (KCl), potassium thiocyanate (KSCN), sodium dihydrogen phosphate (NaH₂PO4), sodium sulfate (Na₂SO₄), sodium chloride (NaCl), sodium hydrogen carbonate (NaHCO3), urea (CO(NH₂)₂), α-amylase (930 U mg⁻¹ A3403), hydrochloric acid (HCl), sodium hydroxide (NaOH), formic acid (HCOOH), pepsin A (674 U mg⁻¹ P7000), pancreatin (762 U mg⁻¹ P1750), bile salts (B8631), and phosphate buffer saline (PBS, pH 7.5) were purchased from Sigma-Aldrich (Madrid, Spain). Methanol and ethyl acetate were supplied by Fisher Scientific (Madrid, Spain). Deionized water was purchased from a Milli-Q water purification system (Millipore, Bedford, MA, USA). LAB used in this study (L. plantarum CECT 220) was obtained from the Spanish Type Culture Collection, CECT, Science Park of the University of Valencia (Paterna, València, Spain). The cultures were kept at −80 °C in glycerol 25% until their use.