Facs 2

Manufactured by BD

Sourced in United States

The BD FACS II is a flow cytometry instrument designed for analyzing and sorting cells. It utilizes laser technology to detect and measure various physical and fluorescent characteristics of cells or particles suspended in a fluid stream.

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Lab products found in correlation

Fxcycle pi rnase staining solution, by Thermo Fisher Scientific (1 mentions) Facsdiva, by BD (1 mentions) Tcs sp5, by Leica (1 mentions) Nano z, by Malvern Panalytical (1 mentions)

Close spelling variants of this product

BD FACS C-Aria II Cell Sorter BD FACS Aria II cell sorted FACS Calibur II flow cytometer BD FACS Canto II 3L BD FACS Jazz™ II Cell Sorter LSR II FACS machine BD FACS Count II Flow Cytometer FACS Aria II or III FACS Arial II FACS Fortessa II flow cytometer

3 protocols using facs 2

Analyzing Cell Cycle in Liver Cancer

Cited 1 time

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Liver cancer cells were seeded at a density of 3×10⁵ cells/well into 6-well plates and cultured for 24 h. Subsequently, cells were transfected with control siRNA or an siRNA targeting human STEAP1, and incubated for 72 h. After incubation, floating cells in media were collected and adhesive cells were washed, fixed in ethanol, and stained with propidium iodide using a cell-cycle analysis kit (FxCycle PI/RNase Staining Solution; Thermo Fisher Scientific), followed by analysis on a BD FACS II (BD Biosciences) instrument using FACSDiva (BD Biosciences) as previously described (20 (link)).

Iijima K., Nakamura H., Takada K., Hayasaka N., Kubo T., Umeyama Y., Iyama S., Miyanishi K., Kobune M, & Kato J. (2021). Six-transmembrane epithelial antigen of the prostate 1 accelerates cell proliferation by targeting c-Myc in liver cancer cells. Oncology Letters, 22(1), 546.

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Nanocomplex Cellular Uptake Evaluation

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Cellular uptake of the nanocomplexes were evaluated in HSC-T6, LX-2 and primary rat HSCs (the 5^th and 16^th passage generations) using flow cytometry and confocal microscopy. The 5’ end of the siRNA antisense strand was labeled with Alex Flour 647. The cells were incubated with the nanocomplexes at 37 °C for various time intervals and then washed with Opti-MEM containing 1mg/mL heparin to remove nonspecifically bound nanocomplexes.
Following heparin treatment, the cells were detached with 0.25% trypsin, washed, suspended, and subjected to fluorescence analysis using a BD FACS II flow cytometer (BD instruments, NJ). For confocal analysis, the cells were stained with 150 nM LysoTracker, fixed with 10% formalin, and examined under a confocal microscope (Leica TCS SP5, Germany).

Zhao Z., Li Y., Jain A., Chen Z., Liu H., Jin W, & Cheng K. (2017). Development of a Peptide-Modified siRNA Nanocomplex for Hepatic Stellate Cells. Nanomedicine : nanotechnology, biology, and medicine, 14(1), 51-61.

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Thiolated Nano-vaccine Characterization

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Scanning electron microscope (SEM) was performed using the Nova NanoSEM 230 (USA) to characterize the morphology and nano size of thiolated nano‐vaccine. Nano ZS (Malvern Instruments, Malvern UK) was used to investigate the DLS of thiolated nano‐vaccine. Zeta potential measurements were also performed at 25 °C on the Nano ZS. The Uv–vis‐NIR absorbance was performed by a Vis‐NIR spectrometer (Spectro Max M5e, Germany). Flow cytometric analysis was performed using a BD FACS II (USA).

Zhang D., Lin Z., Wu M., Cai Z., Zheng Y., He L., Li Z., Zhou J., Sun L., Chen G., Zeng Y., Li J., Liu J., Yang H, & Liu X. (2021). Cytosolic Delivery of Thiolated Neoantigen Nano‐Vaccine Combined with Immune Checkpoint Blockade to Boost Anti‐Cancer T Cell Immunity. Advanced Science, 8(6), 2003504.

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