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Tsq quantum discovery

Manufactured by Thermo Fisher Scientific
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The TSQ Quantum Discovery is a triple quadrupole mass spectrometer designed for high-performance quantitative and qualitative analysis. It features a compact, modular design and delivers fast, sensitive detection and analysis of a wide range of analytes.

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Lab products found in correlation

Xcalibur 2, by Thermo Fisher Scientific (1 mentions) Apap d4, by Santa Cruz Biotechnology (1 mentions) Ace excel 2 superc18 column, by Phenomenex (1 mentions) Kinetex krudkatcher, by Phenomenex (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Methanol, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Luna c18 column, by Phenomenex (1 mentions) 1100 capillary hplc system, by Agilent Technologies (1 mentions)

4 protocols using tsq quantum discovery

1

HPLC-MS/MS Residue Analysis Protocol

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HPLC-MS/MS detection was used for residue analysis by the TSQ Quantum Discovery mass spectrometer system (Thermo Fisher Scientific, USA) equipped with an electrospray interface. Thermo Fisher Xcalibur 2.0.7 software was used to control the instrument and collect and analyze data.
Li Y.H., Wang X.Y., Hua W, & Zhang H. (2020). Studies on Dissipations and Residues of Indoxacarb under Different Field and Environmental Conditions. Journal of Analytical Methods in Chemistry, 2020, 8874759.
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2

Quantitative APAP Determination in Plasma

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Acetaminophen (APAP) was extracted from plasma by liquid-liquid extraction using acidified HPLC grade methanol (Fisher Scientific, Loughborough, UK). Briefly, 10μL plasma was enriched with 10ng APAP-d4 (Santa Cruz Biotechnology Inc, Heidelberg, Germany) as internal standard and 0.8mL methanol (w/0.2% acetic acid, Sigma Aldrich, Gillingham, UK) was added, vortexed and incubated for 20 min on ice. After centrifugation (3000g, 10 min, 10 oC), the supernatant was dried under nitrogen at 40 0C, reconstituted in mobile phase (0.2 mL water:methanol 65:35 v/v) and re-centrifuged. Chromatographic separation used an Aria CTC autosampler and Allegros pump on an ACE Excel 2 SuperC18 column (150 × 3 mm; 2 μm) protected by a Kinetex KrudKatcher® (Phenomenex, UK) at 20 oC and detected on a TSQ Quantum Discovery triple quadrupole mass spectrometer (MS; Thermo Fisher Scientific, UK). The mobile phase consisted of 0.1% formic acid (Sigma Aldrich) in aqueous (A) and 0.1% formic acid in methanol (B) at a flow rate of 0.3 mL/min. Gradient elution was achieved with a total run time of 9 minutes from 35% to 5% B. APAP eluted at 3.95 min. The MS was operated in positive ion electrospray mode (300 oC, 3 kV). Transitions monitored were m/z 152→110 and m/z 156.1→114.1 for APAP and APAP-d4, respectively.
Dean A., van den Driesche S., Wang Y., McKinnell C., Macpherson S., Eddie S.L., Kinnell H., Hurtado-Gonzalez P., Chambers T.J., Stevenson K., Wolfinger E., Hrabalkova L., Calarrao A., Bayne R.A., Hagen C.P., Mitchell R.T., Anderson R.A, & Sharpe R.M. (2016). Analgesic exposure in pregnant rats affects fetal germ cell development with inter-generational reproductive consequences. Scientific Reports, 6, 19789.
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3

Atorvastatin Pharmacokinetics Analysis

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Plasma concentrations of atorvastatin were determined by liquid chromatography–tandem mass spectrometry (LC–MS/MS) using a TSQ Quantum Discovery mass spectrometer (Thermo Electron, San Jose, CA, USA). The ion transitions monitored were m/z 559.2 → 440. Pharmacokinetic parameters were determined by BA-Calc software (Korea Food and Drug Administration, Korea) using actual sampling times. Plasma concentrations of the terminal phase were fitted to a log-linear line by the least squares method to obtain the elimination rate constant. The area under the plasma concentration curve from hour 0 to infinity (AUC∞) was calculated using a combination of the trapezoidal rule and extrapolation to infinity by the elimination rate constant. The maximum drug concentration in plasma (Cmax) and time to Cmax (tmax) were determined from observed values. Clearance (CL) of atorvastatin was adjusted according to the body weight of each subject. Plasma lipid concentrations were measured with an Hitachi 7600-110 chemistry analyzer (Hitachi, Tokyo, Japan).
Woo H.I., Kim S.R., Huh W., Ko J.W, & Lee S.Y. (2017). Association of genetic variations with pharmacokinetics and lipid-lowering response to atorvastatin in healthy Korean subjects. Drug Design, Development and Therapy, 11, 1135-1146.
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4

Mass Spectrometry Analysis of Synthesized Compounds

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The synthesized compounds were analyzed using a Finnigan TSQ Quantum Discovery instrument (Thermo Electron, San Jose, CA) coupled to an Agilent 1100 Capillary HPLC system. The MS was operated in the negative ion ESI mode. A 250 × 10 mm, 5 μm, C18 Luna column and a Krudkatcher disposable precolumn filter (Phenomenex, Torrance, CA) were used. The column was eluted at 10 μL/min with a linear gradient of CH3CN in 15 mM NH4OAc. CH3CN was brought from 15% to 75% in 30 min, then the organic solvent was held at 75% for 5 min followed by a 14 min reequilibration at 15% CH3CN. The ESI source was set in the negative ion mode as follows: voltage 5 kV; current 50 μA; heated ion transfer tube 330 °C. The metabolites were measured by MS/MS using selected reaction monitoring (SRM) mode. The collision energy for all transitions was 15 eV, and the Ar collision gas pressure was 1.0 mTorr. The MS settings of the ion source were optimized using standard solutions of compounds 6 and 9, 7 and 10, and 8 and 11 to produce maximum MS/MS signals at m/z 356 → m/z 209, m/z 338 → 209 and m/z 354 → 225, respectively.
Cheng G., Zarth A.T., Upadhyaya P., Villalta P.W., Balbo S, & Hecht S.S. (2017). Investigation of the Presence in Human Urine of Mercapturic Acids Derived from Phenanthrene, a Representative Polycyclic Aromatic Hydrocarbon. Chemico-biological interactions, 274, 80-88.
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