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Dsx automated elisa system

Manufactured by Dynex
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The Dynex DSX Automated ELISA System is a laboratory instrument designed for automated enzyme-linked immunosorbent assay (ELISA) processing. The core function of the system is to perform various ELISA-based tests and analyses in a controlled and standardized manner.

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Lab products found in correlation

Immulite 2000 xpi immunoassay system, by Siemens (3 mentions) Cobas integra 400 plus analyzer, by Roche (2 mentions) Cobas integra 400 plus analyser, by Roche (1 mentions) Lithium heparin vacutainer, by BD (1 mentions)

7 protocols using dsx automated elisa system

1

Inflammatory Biomarkers in Cardiovascular Procedures

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Complete blood count and biochemistry will be evaluated at the central laboratory of the County Clinical Emergency Hospital of Tirgu Mures as per routine clinical practice, at baseline.
Inflammatory status will be assessed at baseline, respectively at 1-year post procedure. hsCRP levels will be assayed by immunoturbidimetric assay (COBAS INTEGRA 400 plus Analyser, Roche Diagnostics, Switzerland). Matrix metalloprotease 9 and interleukin-6 levels will be assessed via enzyme immunoassay (ELISA) method (Dynex DSX Automated ELISA System, Dynex Technologies, USA; IMMULITE 2000 XPi Immunoassay System, Siemens, USA). Determination of all inflammatory biomarkers will be performed at the Advanced Medical and Pharmaceutical Research Center of the University of Medicine and Pharmacy Targu-Mures. NT-proBNP serum levels will be determined prior to the procedure, at 24 hours, and repeated after 1 year, at the central laboratory of the Emergency Clinical County Hospital of Tirgu-Mures using an electrochemiluminescent immunoassay (IMMULITE 2000 XPi Immunoassay System, Siemens, USA).
Korodi S., Toganel R., Benedek T., Hodas R., Chitu M., Ratiu M., Kovacs I., Mester A, & Benedek I. (2019). Impact of inflammation-mediated myocardial fibrosis on the risk of recurrence after successful ablation of atrial fibrillation – the FIBRO-RISK study: Protocol for a non-randomized clinical trial. Medicine, 98(9), e14504.
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2

Biomarkers of Inflammatory Status

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At baseline, electrochemiluminescent immunoassay (IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America), biochemistry, blood count and NT-proBNP will be determined. Also, inflammatory status will be determined and analyzed at the Advanced Medical and Pharmaceutical Research Center of the University of Medicine and Pharmacy Tirgu-Mures, Romania, based on serum high sensitive C-reactive protein levels determined using immunoturbidimetric assay (COBAS INTEGRA 400 plus analyzer, Roche Diagnostics, Switzerland).
MMP-9 and Il-6 levels will be also evaluated using ELISA method (Dynex DSX Automated ELISA System, Dynex Technologies; IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America).[20 ]
Stanescu A.G., Benedek I., Opincariu D., Hodas R., Ratiu M, & Benedek T. (2021). Assessment of lesion-associated myocardial ischemia based on fusion coronary CT imaging – the FUSE-HEART study: A protocol for non-randomized clinical trial. Medicine, 100(14), e25378.
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3

Biomarkers of Inflammatory Status

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At baseline, electrochemiluminescent immunoassay (IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America), biochemistry, blood count and NT-proBNP will be determined. Also, inflammatory status will be determined and analyzed at the Advanced Medical and Pharmaceutical Research Center of the University of Medicine and Pharmacy Tirgu-Mures, Romania, based on serum high sensitive C-reactive protein levels determined using immunoturbidimetric assay (COBAS INTEGRA 400 plus analyzer, Roche Diagnostics, Switzerland).
MMP-9 and Il-6 levels will be also evaluated using ELISA method (Dynex DSX Automated ELISA System, Dynex Technologies; IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America).[20 ]
Stanescu A.G., Benedek I., Opincariu D., Hodas R., Ratiu M, & Benedek T. (2021). Assessment of lesion-associated myocardial ischemia based on fusion coronary CT imaging – the FUSE-HEART study: A protocol for non-randomized clinical trial. Medicine, 100(14), e25378.
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4

Bovine Anti-Müllerian Hormone Evaluation

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Each herd was visited once per week during the first 6 wk of the breeding season. A single blood sample was collected between 7 and 13 d after first IDE from the coccygeal blood vessels using evacuated blood tubes (lithium heparin Vacutainers; Becton Dickinson and Co., Franklin Lakes, NJ) for AMH determination. After collection, plasma tubes were stored in a cooler box containing ice packs until return to the laboratory (1 to 4 h) and then centrifuged at 1,500 × g for 20 min at 4°C; plasma was then harvested and frozen at -20°C until assayed for AMH. Plasma concentrations (pg/mL) of AMH were analyzed using the Ansh Labs (Webster, TX) Bovine AMH ELISA using a Dynex DSX Automated ELISA System (Dynex Technologies, Chantilly, VA). The assay has an analytical measurable range of 13.5 to 2,240 pg/mL. The AMH assay's lowest detection limit is 11 pg/mL, and intra-and interassay coefficients of variation (CV) were <5%.
Gobikrushanth M., Purfield D.C., Canadas E.R., Herlihy M.M., Kenneally J., Murray M., Kearney F.J., Colazo M.G., Ambrose D.J, & Butler S.T. (2019). Anti-Müllerian hormone in grazing dairy cows: Identification of factors affecting plasma concentration, relationship with phenotypic fertility, and genome-wide associations. Journal of dairy science, 102(12).
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5

SARS-CoV-2 Spike Protein IgG/IgA ELISA

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ELISA plates (Corning #3690) were coated with purified SARS-CoV-2 spike protein and washed and blocked as described above. Aliquots from the samples used for development of the research assay (see above) were diluted manually using 1% milk PBS-T as the diluent to obtain the targeted single dilution for IgG (1:1000) or IgA (1:200). Each of these was added in coated ELISA wells by the DSX automated ELISA system (Dynex Technologies; Chantilly VA) using disposable pipette tips. Incubations, washings, and addition of the anti-human IgG and IgA HRP conjugates, as well as the addition of substrate and stop solution was also accomplished using the automated system. Wash buffer and all reagents were identical to those used for the research assay described above. Reading was accomplished using a dual wavelength mode with the same test wavelength (450 nm) as the research assay and a reference wavelength of 620 nm.
Bortz RH I.I.I., Florez C., Laudermilch E., Wirchnianski A.S., Lasso G., Malonis R.J., Georgiev G.I., Vergnolle O., Herrera N.G., Morano N.C., Campbell S.T., Orner E.P., Mengotto A., Dieterle M.E., Fels J.M., Haslwanter D., Jangra R.K., Celikgil A., Kimmel D., Lee J.H., Mariano M., Antonio N., Jose Q., Rivera J., Szymczak W.A., Tong K., Barnhill J., Forsell M.N., Ahlm C., Stein D.T., Pirofski L.A., Goldstein D.Y., Garforth S.J., Almo S.C., Daily J.P., Prystowsky M.B., Faix J.D., Fox A.S., Weiss L.M., Lai J.R, & Chandran K. (2020). Development, clinical translation, and utility of a COVID-19 antibody test with qualitative and quantitative readouts. medRxiv.
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6

Quantifying HMGB-1 Levels in Sera

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Collected sera was analyzed for high mobility group box-1 (HMGB-1) protein using a Rat HMGB-1 ELISA kit (#50155150, Fischer HealthCare, Inc.). Samples were assayed undiluted on a DSX® Automated ELISA System (Dynex Technologies, Inc., Chantilly, VA, USA) per kit manufacturer's instructions.
Weaver AJ J.r., Brandenburg K.S., Smith B.W, & Leung K.P. (2020). Comparative Analysis of the Host Response in a Rat Model of Deep-Partial and Full-Thickness Burn Wounds With Pseudomonas aeruginosa Infection. Frontiers in Cellular and Infection Microbiology, 9, 466.
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7

Standardized Serum Sample Processing

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Sample management was standardized (Supplemental material S2). Aliquots were transferred on dry ice by batches to the accredited collaborating laboratory (MVZ Dr Eberhard & Partner Dortmund, Dortmund, Germany). Analyses were performed with the same charge of an enzyme-linked accredited immunosorbent assay (EIA) using Dynex DSX automated ELISA system. Assay detection limit was 0.1 mg/L and lower and upper limits of quantification were 0.5 and 12 mg/L, respectively. Sera exceeding the upper limit of quantification were diluted 1:4, thus creating an upper limit of 48 mg/L.
Welzel T., Golhen K., Atkinson A., Gotta V., Ternant D., Kuemmerle-Deschner J.B., Michler C., Koch G., van den Anker J.N., Pfister M, & Woerner A. (2024). Prospective study to characterize adalimumab exposure in pediatric patients with rheumatic diseases. Pediatric Rheumatology Online Journal, 22, 5.
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