The ecdysteroid titers of larvae were measured using the 20-hydroxyecdysone Enzyme Immunoassay (EIA) kit, (Cayman Chemicals) which detects both ecdysone and 20-hydroxyecdysone. Briefly, frozen larvae were homogenized in methanol and ecdysteroids were extracted as described previously [71 (link)]. The extracts were evaporated in a Speed Vac and the residue resuspended in EIA buffer (Cayman Chemical) and analyzed following the manufacturer’s protocol. A standard curve was determined using a dilution series containing a known amount of purified 20-hydroxyecdysone solution provided by the kit. Absorbance at 415 nm was detected using a benchtop microplate reader (Bio-Rad).
20 hydroxyecdysone enzyme immunoassay eia kit
Manufactured by Cayman Chemical
The 20-hydroxyecdysone Enzyme Immunoassay (EIA) kit is a laboratory tool used for the quantitative measurement of 20-hydroxyecdysone, a steroid hormone, in various sample types. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.
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4 protocols using 20 hydroxyecdysone enzyme immunoassay eia kit
1
Quantifying Larval Ecdysteroid Levels
2
Quantification of Larval Ecdysteroids
3
Quantification of Larval Ecdysteroids
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The ecdysteroid titers of larvae were measured using the 20-hydroxyecdysone Enzyme Immunoassay (EIA) kit (Cayman Chemicals), which detects both ecdysone (E) and 20-hydroxyecdysone (20E). Briefly, frozen larvae were homogenized in methanol and ecdysteroids were extracted as described previously (Warren et al., 2006 (link)). The extracts were evaporated in a Speed Vac and the residue resuspended in EIA buffer and analyzed following the manufacturer’s protocol. A standard curve was determined using a dilution series containing a known amount of purified 20E solution provided by the kit. Absorbance at 415 nm was detected using a benchtop microplate reader (Bio-Rad).
4
Ecdysteroid Quantification in Larvae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ecdysteroid titers of larvae were measured using the 20-hydroxyecdysone Enzyme Immunoassay (EIA) kit (Cayman Chemicals), which detects both ecdysone (E) and 20-hydroxyecdysone (20E). Briefly, frozen larvae were homogenized in methanol and ecdysteroids were extracted as described previously (Warren et al., 2006) . The extracts were evaporated in a Speed Vac and the residue resuspended in EIA buffer and analyzed following the manufacturer's protocol. A standard curve was determined using a dilution series containing a known amount of purified 20E solution provided by the kit. Absorbance at 415 nm was detected using a benchtop microplate reader (Bio-Rad).
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