The UV-vis absorption spectra were recorded on a spectrometer (Shimadzu, UV-1700 Pharma Spec UV-vis) over the range of 190–1100 nm in a quartz cuvette (1 cm). The different concentrations of the dispersion were prepared by serial dilutions of a stock dispersion prepared by re dispersing the lyophilized powder in DI water. Photoluminescence measurements were carried out with Fluorolog HORIBA Jobin Yvon, USA spectrofluorometer.
Uv 1700 pharma spec uv vis
Manufactured by Shimadzu
Sourced in Japan
The UV-1700 Pharma Spec UV-vis is a high-performance UV-visible spectrophotometer designed for pharmaceutical applications. It offers precise and reliable measurement capabilities for a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using uv 1700 pharma spec uv vis
1
UV-vis and Photoluminescence Characterization
2
Quantifying Dissolution of PAR and Lα·H2O
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A range of suspensions containing 2.5 g of PAR and 2.5 g of Lα•H2O were made up using solvent compositions ranging from 100% EtOH to 50/50 % v/v EtOH/H2O in 50 mL Eppendorf conical tubes.
They were sealed well and shaken for one hour at a rate of 200 rpm using an orbital shaker (IKA MTS 2/4, Germany). All samples were then moved to an incubator set at 25 ± 3 °C and left for 12 hours to give the suspension adequate time to settle to the bottom of the vial. Two 20 mL aliquots were taken from the supernatant at 25 °C and filtered (25 mm syringe filter with a 0.45 μm polyethersulfone membrane, Fischer, Ireland) labelling one vial as vial 1 and the other as vial 2. Vial 1 was used to test for the concentration of PAR dissolved in solution using a UV-Vis spectrometer (UV-1700 PharmaSpec UV-VIS, Shimadzu, Japan) and scanned at a wavelength of 248 nm (Rote et al., 2012) . Vial 2 was left for 48 hours in a fume hood at 25 ⁰C to allow the EtOH constituent of the solvent mixture to evaporate and then left for 24 hours in an incubator (Gallenkamp Size 1, UK) set at 60 ⁰C to evaporate the remaining H2O constituent. This evaporation of the solvent resulted in large PAR + Lα•H2O crystals. By subtracting the known weight of PAR found in vial 1 from the weight of the crystallised mixture in vial 2, the amount of dissolved Lα•H2O in the mixture was determined.
They were sealed well and shaken for one hour at a rate of 200 rpm using an orbital shaker (IKA MTS 2/4, Germany). All samples were then moved to an incubator set at 25 ± 3 °C and left for 12 hours to give the suspension adequate time to settle to the bottom of the vial. Two 20 mL aliquots were taken from the supernatant at 25 °C and filtered (25 mm syringe filter with a 0.45 μm polyethersulfone membrane, Fischer, Ireland) labelling one vial as vial 1 and the other as vial 2. Vial 1 was used to test for the concentration of PAR dissolved in solution using a UV-Vis spectrometer (UV-1700 PharmaSpec UV-VIS, Shimadzu, Japan) and scanned at a wavelength of 248 nm (Rote et al., 2012) . Vial 2 was left for 48 hours in a fume hood at 25 ⁰C to allow the EtOH constituent of the solvent mixture to evaporate and then left for 24 hours in an incubator (Gallenkamp Size 1, UK) set at 60 ⁰C to evaporate the remaining H2O constituent. This evaporation of the solvent resulted in large PAR + Lα•H2O crystals. By subtracting the known weight of PAR found in vial 1 from the weight of the crystallised mixture in vial 2, the amount of dissolved Lα•H2O in the mixture was determined.
3
Comprehensive Water Quality Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Water and zooplankton samples were collected monthly during the period April 2015 to March 2016. Subsurface water temperature (Tem), dissolved oxygen (DO), pH and conductivity (CON), were performed in situ using a multi-parameter provided with probes (Model WTW Multi 340i/SET-82362). The water transparency was estimated with a Secchi disc (diameter 25 cm).
The water samples for nutrient determinations Nitrates (N-NO3), nitrites (N-NO2), ammonium (N-NH4) and orthophosphate (P-PO4) were performed in the laboratory from water samples preserved in cold and dark conditions using the colorimetric techniques for the application of spectrophotometric methods (Aminot and Chaussepied, 1983)
The suspended solid matter concentration was determined by measuring the dry weight of the filter before and after filtration of 500 ml of water sample through a Whatman GF/C membrane (Aminot and Chaussepied, 1983).
Sub samples (1liter) for quantification of chlorophyll-a, were filtered using Whatman GF/C filters (0.45 μm pore size filter and 47 mmdiameter) and pigment extraction was performed with 90% acetone (SCOR-UNESCO, 1966). The concentrations were determined by the spectrophotometry (Shimadzu UV-1700 Pharma Spec UV-VIS) based on the absorbance at 663,645 and 630 nm.
The water samples for nutrient determinations Nitrates (N-NO3), nitrites (N-NO2), ammonium (N-NH4) and orthophosphate (P-PO4) were performed in the laboratory from water samples preserved in cold and dark conditions using the colorimetric techniques for the application of spectrophotometric methods (Aminot and Chaussepied, 1983)
The suspended solid matter concentration was determined by measuring the dry weight of the filter before and after filtration of 500 ml of water sample through a Whatman GF/C membrane (Aminot and Chaussepied, 1983).
Sub samples (1liter) for quantification of chlorophyll-a, were filtered using Whatman GF/C filters (0.45 μm pore size filter and 47 mmdiameter) and pigment extraction was performed with 90% acetone (SCOR-UNESCO, 1966). The concentrations were determined by the spectrophotometry (Shimadzu UV-1700 Pharma Spec UV-VIS) based on the absorbance at 663,645 and 630 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!