Vaccine-specific IgG, IgG1, IgG2a and IgA antibody titers were measured by ELISA. In brief, 1 µg ml−1 recombinant HA was coated onto ELISA plates in NaHCO3 buffer, pH9.6. After incubation with serially diluted serum samples, HRP-conjugated goat anti-mouse IgG (NA931V, GE healthcare, dilution 1:6000), IgG1 (A90-105P, Bethyl, dilution 1:10000), IgG2a (61-0220, Life Technologies, dilution 1:2000) or IgA (A90-103P, Bethyl, 1:10000) antibody was added to measure specific subtypes. For C57BL/6 mice, anti-mouse IgG2c (1079-05, Southern Biotech, 1:5000) antibody was used in place of anti-IgG2a antibody.
Anti mouse igg2c 1079 05
Manufactured by Southern Biotech
Anti-mouse IgG2c (1079-05) is a laboratory reagent designed for use in immunological research. It is a secondary antibody that specifically binds to the IgG2c subclass of mouse immunoglobulins. This product can be used in various immunoassays and detection methods that require the identification or quantification of mouse IgG2c.
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Lab products found in correlation
2 protocols using anti mouse igg2c 1079 05
1
Vaccine-induced Antibody Titers Quantification
2
Vaccine-induced Antibody Titers Quantification
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